Protein Info for AO356_26685 in Pseudomonas fluorescens FW300-N2C3

Annotation: phosphate import ATP-binding protein PstB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 1 to 253 (253 residues), 385 bits, see alignment E=6.7e-120 PF00005: ABC_tran" amino acids 18 to 169 (152 residues), 98.7 bits, see alignment E=4.8e-32

Best Hits

Swiss-Prot: 97% identical to PSTB1_PSEPK: Phosphate import ATP-binding protein PstB 1 (pstB1) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 99% identity to pfs:PFLU3315)

MetaCyc: 58% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W8V4 at UniProt or InterPro

Protein Sequence (253 amino acids)

>AO356_26685 phosphate import ATP-binding protein PstB (Pseudomonas fluorescens FW300-N2C3)
MDCKLDKIFYGNFMAVRDSHVPIEKNKITGFIGPSGCGKSTVLRSLNRMNDLVKGFRFEG
HVHFLGQDVYGKGVDPVVVRRYIGMVFQQPNPFSMSIFDNVAFGLRLNRYKGDIGDRVKH
ALQGAALWDEVKDKLKVSGLSLSGGQQQRLCIARAIATEPEVLLLDEPCSALDPIATRRV
EELMVELKKDYTIALVTHNMQQAIRVADTTAFFSVDISQGTRTGYLVEMGPTTQIFDNPR
EQMTGDYISGKFS