Protein Info for AO356_26670 in Pseudomonas fluorescens FW300-N2C3

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 PF13185: GAF_2" amino acids 47 to 170 (124 residues), 52 bits, see alignment E=2.8e-17 PF01590: GAF" amino acids 47 to 170 (124 residues), 36.3 bits, see alignment E=2.3e-12 TIGR00229: PAS domain S-box protein" amino acids 180 to 303 (124 residues), 63.7 bits, see alignment E=1.8e-21 PF00989: PAS" amino acids 185 to 293 (109 residues), 31.1 bits, see alignment E=6.3e-11 PF08448: PAS_4" amino acids 194 to 297 (104 residues), 44 bits, see alignment E=7e-15 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 304 to 466 (163 residues), 144.2 bits, see alignment E=3.1e-46 PF00990: GGDEF" amino acids 307 to 464 (158 residues), 157.8 bits, see alignment E=6.3e-50 PF00563: EAL" amino acids 483 to 719 (237 residues), 267.6 bits, see alignment E=2.7e-83

Best Hits

KEGG orthology group: None (inferred from 81% identity to ppu:PP_3319)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WD40 at UniProt or InterPro

Protein Sequence (736 amino acids)

>AO356_26670 histidine kinase (Pseudomonas fluorescens FW300-N2C3)
MNTRPSFHSKTFPESQRDILGMIATDHHLGDILCAICQMHDAQAPETFCSILLTDAEGKR
LLSGAAPSLPVEYSDAIHGMAIGPHEGTCGIAAFRRELVVTEDIARDPNWERFRGLALGH
NLRSCWSVPLLSHLGGVLGTFALYQNRTNAPDEAQIQRLACAAQLAVIAIRHERDGQRLE
ESEQRFRSLFTYNPNPVFALDLAGNIQSVNPAGLKLKPHTASDFIGHHFSHLVLEEDLER
VSQHFSAARAGEPQRFEARVLDESQKLLTMDISNLPIMVNGEIVGVFGIARDISEQKHFE
RQLSFNASHDRLTGLLNRVSLEDRLVLDCHISRRRKRRLAVMCIDLDGFKSINDSIGHYF
GDQVLIEVAQRMPRQVRPGDTIVRMGGDEFIVLLPDLLRDEDVVPVVERLMASIARPYCI
QGIDLHVSASIGITLSDGHIEQPMQLIQQADMAMYKAKQQGRNNFQWYTSDLYQRVCEHA
SLRNDLQKAIETQSLQLHYQPQIDARTGRVVGIEALLRWEHPEKGFISPAVFVPVAEDSG
QIIPLSLWVLDTACAQLRQLGEQGITGISMAVNISPMHFQRGHFVQYVQATLEKHGLCGE
QLELEITESLLLHNAEQAIDTLHRLKALGVCIALDDFGTGFSSLSYLKRLPIDKIKIDRS
FIQEIATDHHDAAITQGIISMAHHLSLTVVAEGVETASQVDFLKGSRCDVFQGYYFAKPM
PYADIEAFLSHPASHP