Protein Info for AO356_25460 in Pseudomonas fluorescens FW300-N2C3

Annotation: sulfonate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13379: NMT1_2" amino acids 55 to 269 (215 residues), 41.8 bits, see alignment E=5.8e-15

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 91% identity to pfo:Pfl01_2205)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X334 at UniProt or InterPro

Protein Sequence (335 amino acids)

>AO356_25460 sulfonate ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2C3)
MAFKRSARTLLVSLAATALLNPLAHAEGKISIAQQFGIGYLILDVVRDQQLIEKHGKAQG
LDIKVDWNSISGATAMNEALLTGALDVVSAGVPPMLTIWDRTRGKQNVKAIASLGSMPNY
LLTNNPNVKSLKDFTDKDRIAVPAAGVGFQSRTLQIETAKVFGNDHFKKFDDISVSLPHP
DATAALIAGQSEINSHFSSPPFQYQALQSPNVHKVLSSYDVLGGPATFNVLYTTEKFHDE
NPKTYKAFYDALVEAQGIIKADKPAAAQTYIRVEQSKLPLALVEKIVTDPEIDFTVVPQR
TYIYAEKLHELGVLKNKADSWKDYFFEEAHADAGS