Protein Info for AO356_25190 in Pseudomonas fluorescens FW300-N2C3

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 163 to 171 (9 residues), see Phobius details PF13675: PilJ" amino acids 34 to 126 (93 residues), 43.5 bits, see alignment E=6e-15 PF00672: HAMP" amino acids 180 to 227 (48 residues), 50.7 bits, see alignment 3.6e-17 PF07730: HisKA_3" amino acids 390 to 455 (66 residues), 50.2 bits, see alignment E=6.1e-17 PF02518: HATPase_c" amino acids 498 to 586 (89 residues), 49.5 bits, see alignment E=1e-16

Best Hits

KEGG orthology group: K07673, two-component system, NarL family, nitrate/nitrite sensor histidine kinase NarX [EC: 2.7.13.3] (inferred from 96% identity to pba:PSEBR_a3151)

Predicted SEED Role

"Nitrate/nitrite sensor protein (EC 2.7.3.-)" in subsystem Nitrate and nitrite ammonification (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H2X7 at UniProt or InterPro

Protein Sequence (599 amino acids)

>AO356_25190 histidine kinase (Pseudomonas fluorescens FW300-N2C3)
MMGWLRSSLPARAGLAVILIAILALASSLSAGLIAWFSQGDAAAINTAGSVRMETYHLSW
KLAAGANDDIPAIIDSLQQRLDSPALRAVLEDGPETSLHKSYRDLIQRWNQTLRPAIERG
DSAFFQSSAPSFVEQLDQFVTLLQRQSEHKQGWQQTIQGMALFSTMIILLIGLYELQYGV
VTPLQELVDATQRFRRGEFQVRVNHQSEDELGQLATSFNTMAETIEQSHRTLENQVQQKT
LNLQQANAALELLYQSSRSLATRQANAEGLDELIRRFQQRLPGLRLTLCLQGQLQAPARH
LLALHGAASRQVCASSDCASCHRHNAARPQAFSISNQGTELGELKAHFVDGHAAQPWETA
LIQALANLIGTSLSLKRQREQDHRLLLLDERTIIARELHDSLAQALSYMKLQVSRMQTLM
RRGEPVQTLETVTGELREGLNNAYRQLRELLTTFRLQIHDDGLVEELKDTAEEFSNRGDF
QVHLFVDTLAFELSASEQIHILQITREALSNCLRHAHAENAWLELRQEGETVRLSIEDDG
RGFSGEVDQREHHGLNIMNERARSLHGQLQIHSRTPQGTRIQVHFQPEFLGQPTEGIAT