Protein Info for AO356_24765 in Pseudomonas fluorescens FW300-N2C3

Annotation: type II secretion system protein GspI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 125 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details PF07963: N_methyl" amino acids 7 to 29 (23 residues), 39 bits, see alignment 3.8e-14 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 7 to 30 (24 residues), 34.1 bits, see alignment 1.6e-12 TIGR01707: type II secretion system protein I" amino acids 9 to 105 (97 residues), 72.8 bits, see alignment E=2.7e-24 PF02501: T2SSI" amino acids 43 to 121 (79 residues), 57.4 bits, see alignment E=1.3e-19

Best Hits

KEGG orthology group: K02458, general secretion pathway protein I (inferred from 98% identity to pba:PSEBR_a3233)

Predicted SEED Role

"General secretion pathway protein I"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X4F5 at UniProt or InterPro

Protein Sequence (125 amino acids)

>AO356_24765 type II secretion system protein GspI (Pseudomonas fluorescens FW300-N2C3)
MPRPAPQAGFTLIEVLVALAIIAVAMSAAVRVAAVMTQSNGLLRDKSIALLAARSQLAQL
RLEGRFAQGSKVFECDQGRLLLRCEQTLRPAENGRLLRVELSVSDRSREAPPLARLETLV
SRDKP