Protein Info for AO356_24440 in Pseudomonas fluorescens FW300-N2C3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 PF01590: GAF" amino acids 27 to 157 (131 residues), 71.4 bits, see alignment E=1.7e-23 PF13185: GAF_2" amino acids 27 to 154 (128 residues), 38.5 bits, see alignment E=2.1e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 162 to 317 (156 residues), 128.8 bits, see alignment E=8.5e-42 PF00990: GGDEF" amino acids 164 to 317 (154 residues), 125.8 bits, see alignment E=2.2e-40

Best Hits

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a3294)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X2J7 at UniProt or InterPro

Protein Sequence (320 amino acids)

>AO356_24440 diguanylate cyclase (Pseudomonas fluorescens FW300-N2C3)
MLAPGKPDNEAVRLKNLHSLKLLDTAPEERFDRLTRLARRLFDVPIALVSLVDANRQWFK
SSAGLDASETPREVSFCGHAILQDQILEICDAEQDERFHDNPLVTDTPGIRFYAGHPLGL
EDGSKLGTLCLLDTKPRQLNDEERELLRDLARMAEQEMVAVQMASMDELTLLSNRRGFKT
LAQHALNVCDRLSRPATLLFFDLNDFKPINDRYGHAEGDSALKTFADVLRIAFRESDVIG
RLGGDEFVALLTGSSYVETSAIMARLKEILDERNAMLQRGYDIRFSVGQIEYDAQRHSSI
DNLLADADAAMYAQKQALHR