Protein Info for AO356_24270 in Pseudomonas fluorescens FW300-N2C3

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1020 transmembrane" amino acids 476 to 485 (10 residues), see Phobius details amino acids 596 to 616 (21 residues), see Phobius details PF00668: Condensation" amino acids 30 to 307 (278 residues), 55.9 bits, see alignment E=6.6e-19 PF00501: AMP-binding" amino acids 421 to 762 (342 residues), 273.5 bits, see alignment E=5.2e-85 TIGR01733: amino acid adenylation domain" amino acids 441 to 834 (394 residues), 402.4 bits, see alignment E=9.9e-125 PF13193: AMP-binding_C" amino acids 818 to 892 (75 residues), 43 bits, see alignment 1.4e-14 PF00550: PP-binding" amino acids 919 to 979 (61 residues), 45.1 bits, see alignment 1.9e-15

Best Hits

KEGG orthology group: None (inferred from 93% identity to pba:PSEBR_a2392)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X7P0 at UniProt or InterPro

Protein Sequence (1020 amino acids)

>AO356_24270 non-ribosomal peptide synthetase (Pseudomonas fluorescens FW300-N2C3)
MSGLQGFRISPQQASVYPHINGAAEHPFSCHLQWSLSQPQDIDALNKRLQALVADSEILR
TRLMPVPGLRHPVQVIDETAPLAVKLQDLRQYNHADQDAALAQLADQPRDWTSPLYVTLV
RLSDEQTILDLAAASSHFDLASLKWLAGALLQDSATDLSDTLQYADYAEWRWSLVEDAPD
HPGVSFWKPSGETEQATLQLSLESILEGGFAPTRLDLALPADLARHPALTPDVLLAAWAA
VLGRLAGQQQVPLTLIHESRGAELENTLGLFEQCLPVNIELDAQVTLLEQSAQVAATLER
AVGWQDYYTGTLDAGYAFAWRSGDARERHASAVMNPLKACLNVRQLPDGLHAQLVYDRHA
LSAEAAGCLAEQWLQLLQGALAEPQQAWAQLPLGGALQSQLIGAGASVPSIEPVTLVELI
ARQVRQQPNAIALSDQDGDLSYSRLDACATQLAHRLVAAGIGPGVPVGILFARSNAAIVA
MLAVLKAGGAYVPVDPTYPADRRAYMLADSAIAHIVVSAELADSVPDHLQRFVLDDLATP
DLGDWPALPLPDANDLAYLIYTSGSTGAPKAVEISHGALSFSTQVRMAAYETPVRAYLLL
SSFAFDSSVAGIFWTLAQGGRLVLPAPGEELEMARLAQLIAQHQVSHGLSLPSLYQALLD
QLQRSDAAAPLACWIVAGEACPPSLIAQHSKTLPHARLVNEYGPTEATVWATYEVLEPSR
NVSIGRPIAGMDLRLLNEFDRACGIGEPGEIVLAGPTLARGYRNKPEETAKAFVTLADGT
RAYRTGDLACWLADGRLAFLGRKDHQVKVRGYRIELGEIERQLCSHSDVREAAVIVQEHA
AGKRLLAYILAAHGYAPDSEAIKRFLGERLPSYMVPALVLTLASFPRTPNGKLDTRQLPD
PDQFDENAHVAPRNAMETALQAIVAKVLKLSTVSVTDNFFAIGGDSILSLQVVAQAHEQG
IALSAKQVFERQTIAAMAEVAQPLEPSAAEPQADSTEFSASGLSDDEMQALMAELDEADL