Protein Info for AO356_22945 in Pseudomonas fluorescens FW300-N2C3

Annotation: peptidase M4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF16485: PLN_propep" amino acids 8 to 48 (41 residues), 52.8 bits, see alignment 4.1e-18 PF01447: Peptidase_M4" amino acids 94 to 177 (84 residues), 105 bits, see alignment E=7.5e-34 PF02868: Peptidase_M4_C" amino acids 180 to 347 (168 residues), 186.1 bits, see alignment E=7.1e-59

Best Hits

Swiss-Prot: 67% identical to PRT1_PECCC: Extracellular metalloprotease (prt1) from Pectobacterium carotovorum subsp. carotovorum

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a3388)

Predicted SEED Role

"Extracellular metalloprotease precursor (EC 3.4.24.-)" (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WM88 at UniProt or InterPro

Protein Sequence (353 amino acids)

>AO356_22945 peptidase M4 (Pseudomonas fluorescens FW300-N2C3)
MIDSRPHHGFIPPYILNRIIAHGSEQQRSSALGTLTHVRNLRHNPGPPSTPPVAAALPKS
GKAGHPQRSVHDAQNSMELPGQPVRLEGQRASGDPAVDEAYDALGATYDFFWKVLGRDSI
DNKGFALVGSVHYGQGYENAFWNGAQMVFGDGDGEIFQRFTRSLDVVAHELAHGVTESEA
GLIYANQSGALNESISDVFGVLVKQFVLEQTADQADWLIGADLLTDKINGDGLRSMSNPG
SAYDDPLLGKDPQPAHMREFVITREDNGGVHINSGIPNRAFYLVATTLGGFAWEKAGRIW
YDTLCDRRLANDASFSAFARLTVEHARQRFGTREVQAVQNGWAQVGVDLTQES