Protein Info for AO356_22725 in Pseudomonas fluorescens FW300-N2C3
Annotation: DNA topoisomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 93% identity to pba:PSEBR_a3431)Predicted SEED Role
No annotation
Isozymes
Compare fitness of predicted isozymes for: 5.99.1.2
Use Curated BLAST to search for 5.99.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9X2Q9 at UniProt or InterPro
Protein Sequence (345 amino acids)
>AO356_22725 DNA topoisomerase (Pseudomonas fluorescens FW300-N2C3) MPDTLPPDALPADLHYVDDTAPGITRKKLRGKFCYFDPQGQRITDAAEIQRINALAVPPA YTDVWICTDPRGHLQATGRDARGRKQYRYHARWREVRDADKYSRMLEFGRALPRLRKRLE EILAKPGFSRDKVMATVITLLDLTLIRVGNTQYARDNRSYGLTTLRNKHVEVNGSAIAFQ FRGKSGIEHQITVKDRRLARIIKRCQEIPGQNLFQYLDENGERHSVSSSDINAYLKSLTG ADFTAKDYRTWAGSAAALAGLRLLSWETESEAKKHVVEMVRQVARQLGNTPSVCRKCYIH PAVVEAFLLGALRQLPKPRLRKGLSEEDAALAIFLQRMADAAQAC