Protein Info for AO356_22350 in Pseudomonas fluorescens FW300-N2C3

Annotation: potassium transporter KefF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 173 PF02525: Flavodoxin_2" amino acids 2 to 167 (166 residues), 159.8 bits, see alignment E=7.4e-51 PF03358: FMN_red" amino acids 43 to 112 (70 residues), 27 bits, see alignment E=3.1e-10

Best Hits

Swiss-Prot: 62% identical to KEFF_SALTI: Glutathione-regulated potassium-efflux system ancillary protein KefF (kefF) from Salmonella typhi

KEGG orthology group: K11746, glutathione-regulated potassium-efflux system ancillary protein KefF (inferred from 88% identity to pba:PSEBR_a3522)

MetaCyc: 60% identical to regulator of KefC-mediated potassium transport and quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
NAD(P)H dehydrogenase (quinone). [EC: 1.6.5.2]

Predicted SEED Role

"Glutathione-regulated potassium-efflux system ancillary protein KefF" in subsystem Glutathione-regulated potassium-efflux system and associated functions or Potassium homeostasis

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.2

Use Curated BLAST to search for 1.6.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X1E4 at UniProt or InterPro

Protein Sequence (173 amino acids)

>AO356_22350 potassium transporter KefF (Pseudomonas fluorescens FW300-N2C3)
MILIIYAHPYPDKSRVNQQMLKRASSHPDVVIRSLYALYPDFDIDVEAEQRAVEQAELVV
LQHPMYWYSSPPLLKLWIDKVFTHGWAYGKGATALKDKSLLWAVTTGGDQGHFQIGGYPG
FAALAQPLHATAIYCGMRWLEPVVVHGAYAAEADAQHAQIEHYAARLAAWKED