Protein Info for AO356_20175 in Pseudomonas fluorescens FW300-N2C3

Annotation: 3-oxoacyl-ACP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00109: ketoacyl-synt
  • amino acids 4 to 247 (244 residues), 195.7 bits, E=1.1e-61
• TIGR03150: beta-ketoacyl-acyl-carrier-protein synthase II
  • amino acids 4 to 410 (407 residues), 645.1 bits, E=1.9e-198
• PF02801: Ketoacyl-synt_C
  • amino acids 255 to 369 (115 residues), 137.7 bits, E=1.9e-44

Best Hits

Swiss-Prot: 67% identical to FABF_ECO57: 3-oxoacyl-[acyl-carrier-protein] synthase 2 (fabF) from Escherichia coli O157:H7

KEGG orthology group: K09458, 3-oxoacyl-[acyl-carrier-protein] synthase II [EC: 2.3.1.179] (inferred from 99% identity to pba:PSEBR_a4049)

MetaCyc: 67% identical to 3-oxoacyl-[acyl carrier protein] synthase 2 (Escherichia coli K-12 substr. MG1655)
Beta-ketoacyl-acyl-carrier-protein synthase II. [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179 [EC: 2.3.1.179, 2.3.1.41]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179)" (EC 2.3.1.179)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• biotin biosynthesis I (13/15 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• stearate biosynthesis IV (4/6 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• petroselinate biosynthesis (2/6 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.179, 2.3.1.41

Use Curated BLAST to search for 2.3.1.179 or 2.3.1.41

Sequence Analysis Tools

See A0A0N9WBM8 at UniProt or InterPro

Protein Sequence (414 amino acids)

>AO356_20175 3-oxoacyl-ACP synthase (Pseudomonas fluorescens FW300-N2C3)
VSRRRVVVTGMGMLSPLGTDVPSSWQGILAGRSGIGLIEHTDLSAYSTRFGGSVKGFNVE
EYLSVKEARKLDLFIQYGLAAGFQAVRNAGLEVTDANRERIGVAMGSGIGGLTNIEETSR
TLHDSGPRRISPFFVPGSIINMISGFLSIHLGAQGPNYAIATACTTGTHCIGMAARNIMY
DEADVMIAGGAEMAACGLGMGGFGASRALSTRNDEPARASRPWDKGRDGFVLSDGAGALV
LEELEHAKARGATIYAELIGFGTSGDAYHMTSPPADGAGAARCIANALRDAKINGEQVQY
INAHGTSTPAGDLAEAQAIKTVFGEHAYKLAVSSTKSMTGHLLGAAGAVEAIFSVLAINS
QTAPPTINLDEPDDGCDLDFVPHTARSMDIDVVLSNSFGFGGTNGSLVFRRFAG