Protein Info for AO356_19250 in Pseudomonas fluorescens FW300-N2C3

Annotation: glycerol acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 624 transmembrane" amino acids 52 to 72 (21 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 110 to 128 (19 residues), see Phobius details amino acids 147 to 167 (21 residues), see Phobius details amino acids 173 to 191 (19 residues), see Phobius details amino acids 233 to 252 (20 residues), see Phobius details amino acids 264 to 283 (20 residues), see Phobius details amino acids 295 to 313 (19 residues), see Phobius details amino acids 334 to 359 (26 residues), see Phobius details amino acids 377 to 397 (21 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details amino acids 595 to 608 (14 residues), see Phobius details PF07690: MFS_1" amino acids 21 to 387 (367 residues), 47.5 bits, see alignment E=1.2e-16 PF01553: Acyltransferase" amino acids 442 to 570 (129 residues), 91.1 bits, see alignment E=5.5e-30

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_1665)

Predicted SEED Role

"1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Phosphate metabolism (EC 2.3.1.51)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.51

Use Curated BLAST to search for 2.3.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WA72 at UniProt or InterPro

Protein Sequence (624 amino acids)

>AO356_19250 glycerol acyltransferase (Pseudomonas fluorescens FW300-N2C3)
MSHPSQFTLLRTRRFLPFFITQSLGAFNDNIFKQSLILAILYKLTIEGDRSIWVNLCALL
FILPFFLFSALAGQFGEKFAKDALIRLIKLGEIVIMAVGAVGFVFDHLSLMLVALFAMGT
HSALFGPVKYSILPQALHEEELVGGNGLVEMGTFLAILAGTIGAGIMMSSAHYAPVVSTA
IIGVAVLGYLASRGIPRAAAASPHMRLNWNIFSQSWATLKLGLGQTPAVSRSIVGNSWFW
FVGAIYLTQIPAYAKEWMHGDETVVTLILTVFSVGIALGSMLCEKLSGRKVEIGLVPFGS
FGLTVFGLLLWWHSGGIPDSVEGHGWLEVLGFGHAWLVLIDILGLGVFGGFYIVPLYALI
QSRTVEDERARVIAANNILNALFMVVSAIVSIVLLSLAKLSIPQLFLVVSLLNIGVNAYI
FKIVPEFSMRFMIWLLSHSMYRVEHRNLEAIPDEGAALLVCNHVSFVDALLIGGAVRRPI
RFVMYYKIYNLPVLNFIFRTAGTIPIAGRQEDIQIYEKAFKRIAQYLKDGELVCIFPEGK
LTADGEINEFKSGLTRILQETPVPVIPLALQGLWGSFFSRDPAKGLFRRFWSRVTLVAGS
AVAVEAATPSSLQVLVGDLRGTVR