Protein Info for AO356_18360 in Pseudomonas fluorescens FW300-N2C3

Annotation: electron transporter RnfD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 transmembrane" amino acids 14 to 32 (19 residues), see Phobius details amino acids 38 to 58 (21 residues), see Phobius details amino acids 71 to 103 (33 residues), see Phobius details amino acids 114 to 134 (21 residues), see Phobius details amino acids 193 to 209 (17 residues), see Phobius details amino acids 216 to 237 (22 residues), see Phobius details amino acids 244 to 262 (19 residues), see Phobius details amino acids 273 to 292 (20 residues), see Phobius details amino acids 298 to 317 (20 residues), see Phobius details PF03116: NQR2_RnfD_RnfE" amino acids 10 to 320 (311 residues), 302.7 bits, see alignment E=1.5e-94 TIGR01946: electron transport complex, RnfABCDGE type, D subunit" amino acids 10 to 320 (311 residues), 353.4 bits, see alignment E=7e-110

Best Hits

Swiss-Prot: 62% identical to RNFD_PSEMY: Ion-translocating oxidoreductase complex subunit D (rnfD) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K03614, electron transport complex protein RnfD (inferred from 91% identity to pba:PSEBR_a4383)

Predicted SEED Role

"Electron transport complex protein RnfD" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WKC2 at UniProt or InterPro

Protein Sequence (326 amino acids)

>AO356_18360 electron transporter RnfD (Pseudomonas fluorescens FW300-N2C3)
MPLPDTVDERLRPAMTRVLLATLPGVLALLWFQGWGVVLNLLLSGLTALAVEALVLRLRR
RPLKPELNDGSALVSATLLALALPAYCPWWLTVTAAACALLFGKHLWGGVGSNPFNPAML
GYALVLVAFPAHMSPAPHGLGLLEGLQQVFGTHPTPDAWAGATALDSLRTNTSLTIDELF
ASDPAFGRFGGRGAEWVNLAFLAGGLFLLQQRVFSWHAPVGLLGSLFIVSLLCWNGSGSD
SHGSPLFHLFSGATMLGAFFIATEPVSGAKAPLARLLFGAGMGLLVYLIRTWGGYPDGVA
FAVLLMNLGVPALDRFAEARQRRPLT