Protein Info for AO356_17790 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): alpha-ketoglutarate permease (MHS family)
Rationale: Specific phenotype on a-ketoglutarate. Not important for the utilization of other dicarboxylates (succinate or malate). 83% identical to PA5530, which is reported to transport a-ketoglutarate and also glutarate (PMC4097582). KEGG_correct
Original annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 transmembrane" amino acids 39 to 57 (19 residues), see Phobius details amino acids 68 to 88 (21 residues), see Phobius details amino acids 99 to 117 (19 residues), see Phobius details amino acids 124 to 146 (23 residues), see Phobius details amino acids 167 to 187 (21 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 249 to 267 (19 residues), see Phobius details amino acids 286 to 304 (19 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details amino acids 340 to 364 (25 residues), see Phobius details amino acids 376 to 397 (22 residues), see Phobius details amino acids 409 to 427 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 32 to 244 (213 residues), 108.3 bits, see alignment E=4.6e-35 amino acids 224 to 431 (208 residues), 40.6 bits, see alignment E=1.6e-14 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 34 to 420 (387 residues), 443.9 bits, see alignment E=3e-137 PF07690: MFS_1" amino acids 61 to 380 (320 residues), 103.8 bits, see alignment E=9.5e-34

Best Hits

Swiss-Prot: 56% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 99% identity to pba:PSEBR_a4491)

MetaCyc: 56% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Dicarboxylate MFS transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WZ17 at UniProt or InterPro

Protein Sequence (439 amino acids)

>AO356_17790 alpha-ketoglutarate permease (MHS family) (Pseudomonas fluorescens FW300-N2C3)
MDNSNALPIGSAAVPARERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKVFFPKG
DTTAQLLNTAAIFAVGFLMRPIGGWLMGLYADRAGRKRALMASVYLMCFGSLIIALSPSY
ETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKERRGFFSSFQYVTLISGQLIA
LGVLIVLQQFLTTEQLYAWGWRIPFAIGALCAVVALYLRRGMEETESFTKKEKSKESAMR
TLLRHPKELMTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQ
PVIGGLSDKIGRRPILIAFGILGTLFTVPILTTLHTIQTWWGAFFLIMAALIIVSGYTSI
NAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWFKSIGMETGYYWYVTACIAVS
LLVYITMKDTRKHSRIVTD