Protein Info for AO356_17540 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF)
Rationale: Specific phenotype on NAG as a carbon source. Not sure why SEED think s this includes the IIA component. (KEGG_correct)
Original annotation: PTS N-acetyl-D-glucosamine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 10 to 133 (124 residues), 140.2 bits, see alignment E=7.2e-45 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 11 to 130 (120 residues), 123.6 bits, see alignment E=6.6e-40 PF00381: PTS-HPr" amino acids 175 to 251 (77 residues), 73.9 bits, see alignment E=2.3e-24 TIGR01003: phosphocarrier, HPr family" amino acids 175 to 251 (77 residues), 66.4 bits, see alignment E=2.6e-22 PF05524: PEP-utilisers_N" amino acids 279 to 398 (120 residues), 71.7 bits, see alignment E=1.6e-23 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 305 to 826 (522 residues), 453.2 bits, see alignment E=1.8e-139 PF00391: PEP-utilizers" amino acids 423 to 495 (73 residues), 62 bits, see alignment E=8.7e-21 PF02896: PEP-utilizers_C" amino acids 521 to 806 (286 residues), 341.7 bits, see alignment E=8.3e-106

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 89% identity to pba:PSEBR_a4545)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WA75 at UniProt or InterPro

Protein Sequence (837 amino acids)

>AO356_17540 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (Pseudomonas fluorescens FW300-N2C3)
MPNNNKELILSAPLSGPVLTLANVPDAVFASGAMGDGIAIDPLNDTLYAPCDAEVIHVAR
TGHAVTLRADNGAELLLHLGLDTVELQGEGFSMLVKEGARVSHGQPLLRYDVDKVALRCK
SLVSLLVITNGEHFQARPITLKGVKVGEPLLHILAKAVGQAEHHDQDSGIEVFGQVRIAH
RGGLHARPAALVRQTAQGFKSRSQLHFSGKSASCDSVMGMMGLAITEQAQVHVSCRGSDA
EAALQALLTTLSTALVEEAHASAPPPEPPRANAEEGVLHGVCAAPGLVTGPLVRLSGIQL
PEDIGGHAIEEQRQRLSDALAQVRGEIHLTLEHARARQHRDEEAIFSAHLALLEDPVLLD
AADLFIEQGSAAPHAWSRSIDTQCQVLQQLGSTLLAERANDLRDLRQRVLRVLLGEAWQF
DVAAGAIVAAQELTPSDLLQLSAQGVAGVCMVEGGATSHVAILARGKGLPCLVALGDELL
AQEQGQAVVLDADGGRLELTPTVERLAQVRQAQTRRTALRAQQQSLAHTPARTVDGVEVE
VAANVASSAEAGESLANGADGVGLLRTEFLFVDRHTAPDEEEQRQAYQAVLEAMGDKPVI
IRTIDVGGDKQLDYLPLPSEANPVLGLRGIRLAQVRPDLLDQQLRALLQTRPLDRCRILL
PMVTEVDELLHIRKRLDALGSELGLSERPQLGVMVEVPAAALLAEQLAEHADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWVGVCGALASDPLATPVL
IGLGVRELSVSPPQIGEIKDRVRHLDAAQCARLSNELLNLGSALAVRRACHRHWPLG