Protein Info for AO356_17370 in Pseudomonas fluorescens FW300-N2C3

Annotation: ATP-dependent RNA helicase RhlB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 PF00270: DEAD" amino acids 122 to 297 (176 residues), 160.3 bits, see alignment E=5.6e-51 PF04851: ResIII" amino acids 142 to 292 (151 residues), 33.5 bits, see alignment E=6e-12 PF00271: Helicase_C" amino acids 333 to 441 (109 residues), 102.4 bits, see alignment E=2.5e-33

Best Hits

Swiss-Prot: 93% identical to RHLB_PSESM: ATP-dependent RNA helicase RhlB (rhlB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03732, ATP-dependent RNA helicase RhlB [EC: 3.6.4.13] (inferred from 98% identity to pba:PSEBR_a4579)

Predicted SEED Role

"ATP-dependent RNA helicase RhlB" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X0J9 at UniProt or InterPro

Protein Sequence (487 amino acids)

>AO356_17370 ATP-dependent RNA helicase RhlB (Pseudomonas fluorescens FW300-N2C3)
MTVLKALKKMFGKSEAEQLAPGSTAPTHGPSRTDAQQPRRTAPVAQAKKEPVAQPVKSPE
PETPRNEAPRPRRERAPKPPVVAWKLEDFAVEPQEGKTRFHDFKLAPELMHAIQDLGFPY
CTPIQAQVLGYTLAGKDAIGRAQTGTGKTAAFLISIITQLLQTPPPKERYMGEPRALIIA
PTRELVVQIAKDAADLTKYTGLNVMTFVGGMDFDKQLKHLEARHCDILVATPGRLLDFNQ
RGDVHLDMVEVMVLDEADRMLDMGFIPQVRQIIRQTPPKSERQTLLFSATFTEDVMNLAK
QWTTDPAIVEIEAENVASENVEQHIYAVAGADKYKLLYNLVNDNGWERVMVFANRKDEVR
RIEERLVRDGVNAAQLSGDVPQHKRIKTLEGFREGKIRVLVATDVAGRGIHIDGISHVIN
FTLPEVPDDYVHRIGRTGRAGAAGVSISFAGEDDSYQLPSIEALLGRKISCEMPPTHLLR
PVERKRP