Protein Info for AO356_17350 in Pseudomonas fluorescens FW300-N2C3

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01096: lysine-arginine-ornithine-binding periplasmic protein" amino acids 7 to 251 (245 residues), 270 bits, see alignment E=9.8e-85 PF00497: SBP_bac_3" amino acids 27 to 252 (226 residues), 212.5 bits, see alignment E=5.6e-67 PF10613: Lig_chan-Glu_bd" amino acids 67 to 118 (52 residues), 36.1 bits, see alignment E=6.2e-13

Best Hits

Swiss-Prot: 46% identical to ARGT_ECOLI: Lysine/arginine/ornithine-binding periplasmic protein (argT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4583)

Predicted SEED Role

"Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein" in subsystem Arginine and Ornithine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WTK0 at UniProt or InterPro

Protein Sequence (258 amino acids)

>AO356_17350 ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2C3)
MSKLPLITGLALSLLASSSLFAAEKTLRIGIEAAYPPFASKTSEGKIEGFDYDIGNALCA
QMQVKCQWIEGEFDGLIPSLKVKKIDLALSSMTITEERKKSVDFTHKYYFTSSRLVMKEG
AVVDDQYASLKGKTVGVQRATTTDRYATEVLEPKGVTVKRYSNNEEIYMDLASGRLDAIF
ADTIPLEDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTQLVADLNKAIDGIRAN
GKYQEIQAKYFKSDIYGD