Protein Info for AO356_17290 in Pseudomonas fluorescens FW300-N2C3

Annotation: poly(beta-D-mannuronate) C5 epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF05048: NosD" amino acids 275 to 419 (145 residues), 51.1 bits, see alignment E=1.2e-17 PF13229: Beta_helix" amino acids 332 to 428 (97 residues), 35.5 bits, see alignment E=7.9e-13 TIGR03804: parallel beta-helix repeat" amino acids 381 to 418 (38 residues), 25.4 bits, see alignment 4.4e-10

Best Hits

Swiss-Prot: 84% identical to ALGG_PSEFL: Mannuronan C5-epimerase (algG) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4595)

MetaCyc: 68% identical to mannuronan C5 epimerase (Pseudomonas aeruginosa)
RXN-9839 [EC: 5.1.3.37]; TRANS-RXN-269 [EC: 5.1.3.37]

Predicted SEED Role

"Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)" in subsystem Alginate metabolism (EC 5.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.- or 5.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJT8 at UniProt or InterPro

Protein Sequence (529 amino acids)

>AO356_17290 poly(beta-D-mannuronate) C5 epimerase (Pseudomonas fluorescens FW300-N2C3)
MNRYLRNSQAMKGSISLLVAAMLLAGSPAFANVEPVVKPGNVVKELQRAKTYTVNSAPTA
PLELAKPTLPDLSGYTAEAVAAKIVRTKPGKVSVRRMMQENALKDFIGGDNKMAEWVVRQ
HGIPQAIFLDDGYMNLKDLAKKAPKYIIETSPGVYLAKIPIVVGQKGILEIDKQTQELRL
SQEGGSFLVNDGHMFIRDTKVTGWREKDNGPATFRSPKEFRPFLLAWGGTETYIVNTKMA
SFGYANSKSYGVSISQYTPNMAKVLKRPEPTGWIIGSEFSDMWYGFYCYETRDFVVKGNT
YKDNIVYGIDPHDRSHRLIIADNTVYGTKKKHGIIVSREVNDSFIFNNRSYDNKLSGLVI
DRNSVNNLIAYNEIYKNHTDGITLYESADNLLWGNKVISNRRHGIRIRNSVNIRLYENVS
MANGLTGIYGHIKDLSDTDRDIKLDPFDAEVSLIVVGGELAANGSGPLSIDSPLSVELYR
VSMLAPTKSSGISFTGILGERQDEILDLLVRQQKAVLIDPVERQTELRD