Protein Info for AO356_15310 in Pseudomonas fluorescens FW300-N2C3

Annotation: penicillin-binding protein 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 20 to 607 (588 residues), 787.8 bits, see alignment E=3.5e-241 PF03717: PBP_dimer" amino acids 63 to 235 (173 residues), 149.7 bits, see alignment E=1.3e-47 PF00905: Transpeptidase" amino acids 268 to 604 (337 residues), 255 bits, see alignment E=9.3e-80

Best Hits

Swiss-Prot: 44% identical to MRDA_ECO57: Peptidoglycan D,D-transpeptidase MrdA (mrdA) from Escherichia coli O157:H7

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 98% identity to pba:PSEBR_a4964)

MetaCyc: 44% identical to peptidoglycan DD-transpeptidase MrdA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Penicillin-binding protein 2 (PBP-2)" in subsystem Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W8X8 at UniProt or InterPro

Protein Sequence (631 amino acids)

>AO356_15310 penicillin-binding protein 2 (Pseudomonas fluorescens FW300-N2C3)
MPQPIRIKDHEKDARLVRGRVVFGAITVVTLIGVLIARLYFLQVIQYEYHSTLSENNRVH
VQPIPPTRGLIFDRNGVVVADNRPSFSLSMTRERSGDWQQVLDVIVEVLQLTPEDRAIFE
KRMRQGRRPFEPVPILFELTEEQIALIAVNQFRLPGVEVVAQLVRHYPQGPHFAHSVGYM
GRINEKELKTLDPVNYSGTHHIGKTGIERFYEPELHGQVGYEEVETNARGRVLRVLKRTD
PIPGKDIVLSLDIKLQEAAEAALGGRRGAVVALDPNTGEVLAMVSQPSFDPNLFVTGISF
KAYAELRDSIDRPLFNRVLRGLYPPGSTIKPAVAIAGLDSGVVTASSRVYDPGYYQLPNY
DHKYRNWNRTGDGYVDLETAIMRSNDTYFYDLAHKLGIDRLSSYMNKFGLGQKVSLDMFE
ESPGLMPSREWKRATRRQAWFPGETLILGIGQGYMQATPLQLAQATALVASKGKWYRPHL
AKTIEGQKPVDPDPMPDIILRNPSDWQKVNNGMQQVMHGARGTARKASIGAQYRIAGKSG
TAQVVAIKQGEKYDRSKVQERHRDHALFVGFAPADNPKIVVAVMVENGESGSGVAAPVVR
QIMDAWLLDEHGQLKPEYASPITAEATAREE