Protein Info for AO356_14610 in Pseudomonas fluorescens FW300-N2C3

Annotation: DNA-directed RNA polymerase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1357 TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 8 to 1353 (1346 residues), 1881 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 27 to 504 (478 residues), 100 bits, see alignment E=4.2e-32 PF04561: RNA_pol_Rpb2_2" amino acids 155 to 227 (73 residues), 32.4 bits, see alignment 2.2e-11 amino acids 360 to 459 (100 residues), 56.3 bits, see alignment 1.1e-18 PF04565: RNA_pol_Rpb2_3" amino acids 518 to 585 (68 residues), 104 bits, see alignment 1.2e-33 PF10385: RNA_pol_Rpb2_45" amino acids 596 to 661 (66 residues), 73.4 bits, see alignment 3.7e-24 PF00562: RNA_pol_Rpb2_6" amino acids 722 to 1279 (558 residues), 458.9 bits, see alignment E=4.4e-141 PF04560: RNA_pol_Rpb2_7" amino acids 1281 to 1356 (76 residues), 98.4 bits, see alignment 7.6e-32

Best Hits

KEGG orthology group: K03043, DNA-directed RNA polymerase subunit beta [EC: 2.7.7.6] (inferred from 63% identity to aaa:Acav_4447)

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WZW7 at UniProt or InterPro

Protein Sequence (1357 amino acids)

>AO356_14610 DNA-directed RNA polymerase subunit beta (Pseudomonas fluorescens FW300-N2C3)
MAYSYTEKKRIRKDFSKLPDVMDVPYLLAIQLDSYREFLQAGATKDQFRDVGLHAAFKSV
FPIISYSGNAALEYVGYRLGEPAFDVKECVLRGVTYAVPLRVKVRLIIFDKESSNKAIKD
IKEQEVYMGEIPLMTENGTFVINGTERVIVSQLHRSPGVFFDHDRGKTHSSGKLLYSARI
IPYRGSWLDFEFDPKDCVFVRIDRRRKLPASVLLRALGYTTEEVLDAFYTTNVFHVQGEN
LSLELVPQRLRGEIAVLDIQDDKGKVIVEQGRRITARHINQLEKAGIKELQVPIDYVLGR
TTAKVIVHPATGEILAECNTELTTEILAKIAKAQVVRIETLYTNDIDCGPFISDTLKIDS
TGNQLEALVEIYRMMRPGEPPTKDAAETLFNNLFFSPERYDLSAVGRMKFNRRIGRTEIE
GSGVLNKDDIVAVLKTLVDIRNGKGIVDDIDHLGNRRVRCVGEMAENQFRVGLVRVERAV
KERLSMAESEGLMPQDLINAKPVAAAVKEFFGSSQLSQFMDQNNPLSEITHKRRVSALGP
GGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLAAYARTNQYGFLESPYRVVK
DALVTDEIVFLSAIEEADHVIAQASATMNDKKVLVDELVAVRHLNEFTVKAPEDVTLMDV
SPKQVVSVAASLIPFLEHDDANRALMGSNMQRQAVPTLRADKPLVGTGMERNVARDSGVC
VVARRGGVIDSVDASRIVVRVADDEVETGEAGVDIYNLTKYTRSNQNTCINQRPLVSKGD
RVQRSDIMADGPSTDMGELALGQNMRIAFMAWNGFNFEDSICLSERVVQEDRFTTIHIQE
LTCVARDTKLGPEEITADIPNVGEAALNKLDEAGIVYVGAEVGAGDILVGKVTPKGETQL
TPEEKLLRAIFGEKASDVKDTSLRVPTGTKGTVIDVQVFTRDGVERDARALSIEKTQLDE
IRKDLNEEFRIVEGATFERLRSALVGHKAEGGAGLKKGQEITDEVLDGLEHGQWFKLRMA
EDALNEQLEKAQAYIVDRRRLLDDKFEDKKRKLQQGDDLAPGVLKIVKVYLAIRRRIQPG
DKMAGRHGNKGVVSVIMPVEDMPHDANGTPVDVVLNPLGVPSRMNVGQILETHLGLAAKG
LGEKINRMIEEQRKVADLRKFLHEIYNEIGGRNEELDTFSDQEILDLAKNLRGGVPMATP
VFDGAKESEIKAMLKLADLPESGQMQLFDGRTGNKFERPVTVGYMYMLKLNHLVDDKMHA
RSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMY
KNIVDGDHRMEPGMPESFNVLIKEIRSLGIDIDLETE