Protein Info for AO356_14270 in Pseudomonas fluorescens FW300-N2C3

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 652 TIGR01391: DNA primase" amino acids 5 to 420 (416 residues), 485.6 bits, see alignment E=6.1e-150 PF01807: zf-CHC2" amino acids 6 to 100 (95 residues), 137.8 bits, see alignment E=3.7e-44 PF08275: DNAG_N" amino acids 126 to 254 (129 residues), 152.1 bits, see alignment E=3.4e-48 PF13662: Toprim_4" amino acids 261 to 330 (70 residues), 63.9 bits, see alignment E=5.3e-21 PF01751: Toprim" amino acids 262 to 339 (78 residues), 54.5 bits, see alignment E=4.3e-18 PF13362: Toprim_3" amino acids 263 to 358 (96 residues), 34.4 bits, see alignment E=1e-11 PF13155: Toprim_2" amino acids 264 to 351 (88 residues), 65.1 bits, see alignment E=2.4e-21 PF10410: DnaB_bind" amino acids 373 to 426 (54 residues), 40 bits, see alignment 1.5e-13 PF08278: DnaG_DnaB_bind" amino acids 517 to 637 (121 residues), 96.7 bits, see alignment E=5.8e-31

Best Hits

Swiss-Prot: 82% identical to DNAG_PSEPK: DNA primase (dnaG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 99% identity to pba:PSEBR_a5150)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W8K9 at UniProt or InterPro

Protein Sequence (652 amino acids)

>AO356_14270 DNA primase (Pseudomonas fluorescens FW300-N2C3)
MAGLIPQSFIDDLLNRTDIVDVVSSRLQMKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY
CFGCGAGGNALGFIMDHDNLDFPQAVEELAKAAGMEIPREESGRPHKPRQPTDSPLYPLL
TAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLGFAPPGWDNLYKHLSSDTLQQ
RAMIDAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPV
FHKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHMKRLF
RVVPNVLFCFDGDQAGRNAAWRALEATLPCLQDGRRARFLFLPEGEDPDTLVRSEGTDAF
RARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAPLIDKVPGANLRTLMRQRLTE
ITGLNSEAVNQLVHSAPQEAPPAYDPGIDYDAMPDFADYHQPQQDYALQQEWTPKKPGSG
GKKWDKKPWDKNGKRGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHFAAE
DHSNTQLLVALLEAVQKNPKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQFFD
TITSLSARQRERNLEQLLRKARQSELTSEEKNQLRDLLSRNVCASNPTSTGA