Protein Info for AO356_14230 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): lysine 2-monooxygenase (EC 1.13.12.2)
Rationale: Specifically important for: L-Lysine. this is the first step in the oxidation of lysine (SEED_correct)
Original annotation: amine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 PF13450: NAD_binding_8" amino acids 50 to 117 (68 residues), 37.2 bits, see alignment E=4.3e-13 PF01593: Amino_oxidase" amino acids 55 to 530 (476 residues), 164.8 bits, see alignment E=6.8e-52

Best Hits

KEGG orthology group: K00466, tryptophan 2-monooxygenase [EC: 1.13.12.3] (inferred from 99% identity to pba:PSEBR_a5163)

MetaCyc: 90% identical to L-lysine monooxygenase monomer (Pseudomonas putida)
Lysine 2-monooxygenase. [EC: 1.13.12.2]

Predicted SEED Role

"Lysine 2-monooxygenase (EC 1.13.12.2)" in subsystem Lysine degradation (EC 1.13.12.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.12.2 or 1.13.12.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WVY4 at UniProt or InterPro

Protein Sequence (560 amino acids)

>AO356_14230 lysine 2-monooxygenase (EC 1.13.12.2) (Pseudomonas fluorescens FW300-N2C3)
MNKNNRHPADGKKPITIFGPDFPFAFDDWIEHPAGLGSIPAHNHGTEVAIVGAGIAGLVA
AYELMKLGLKPVVYEASKMGGRLRSQAFNGAEGVIAELGGMRFPVSSTAFYHYVDKLGLE
TKPFPNPLTPASGSTVIDLEGQTYYAQKLADLPALFQEVADAWADALEDGSRFGDIQQAI
RDRDVPRLKELWNTLVPLWDDRTFYDFVATSKAFAKLSFQHREVFGQVGFGTGGWDSDFP
NSMLEIFRVVMTNCDDHQHLVVGGVEQVPHGIWNHVPERCAHWPEGTSLNSLHLGAPRSG
VKRIARSADGRFSVTDVWENTREYAAVLVTCQSWLLTTQIECEEALFSQKMWMALDRTRY
MQSSKTFVMVDRPFWKDKDPETGRDLMSMTLTDRLTRGTYLFDNGDDKPGVICLSYSWMS
DALKMLPHPVEKRVKLALDALKKIYPKVDIAARIIGDPITVSWEADPHFLGAFKGALPGH
YRYNQRMYAHFMQDDMPAEQRGIFIAGDDVSWTPAWVEGAVQTSLNAVWGIMKHFGGETH
AENPGPGDVFHEIGPIALPE