Protein Info for AO356_13420 in Pseudomonas fluorescens FW300-N2C3

Annotation: pyrroline-5-carboxylate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03807: F420_oxidored
  • amino acids 5 to 99 (95 residues), 77 bits, E=3.5e-25
• PF03446: NAD_binding_2
  • amino acids 5 to 96 (92 residues), 24.7 bits, E=5.3e-09
• PF02558: ApbA
  • amino acids 6 to 100 (95 residues), 23.4 bits, E=1e-08
• TIGR00112: pyrroline-5-carboxylate reductase
  • amino acids 6 to 266 (261 residues), 257.6 bits, E=7.4e-81
• PF01210: NAD_Gly3P_dh_N
  • amino acids 45 to 102 (58 residues), 28.4 bits, E=3.7e-10
• PF14748: P5CR_dimer
  • amino acids 162 to 266 (105 residues), 120.7 bits, E=8e-39

Best Hits

Swiss-Prot: 73% identical to P5CR_PSEAE: Pyrroline-5-carboxylate reductase (proC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00286, pyrroline-5-carboxylate reductase [EC: 1.5.1.2] (inferred from 97% identity to pba:PSEBR_a5322)

Predicted SEED Role

"Pyrroline-5-carboxylate reductase (EC 1.5.1.2)" in subsystem Proline Synthesis (EC 1.5.1.2)

MetaCyc Pathways

• L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
• L-arginine degradation VI (arginase 2 pathway) (3/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.2

Use Curated BLAST to search for 1.5.1.2

Sequence Analysis Tools

See A0A0N9WX95 at UniProt or InterPro

Protein Sequence (272 amino acids)

>AO356_13420 pyrroline-5-carboxylate reductase (Pseudomonas fluorescens FW300-N2C3)
MSNTRIAFIGAGNMAASLIGGLRAKGLDASLIRASDPGEETRTRVSAEHGIETFADNAQA
IDGADVIVLAVKPQAMKTVCEALRPSLKPHQLVVSIAAGITCASMNNWLGEQPIVRCMPN
TPALLRQGVSGLFATPHVSAEQRQQAETLLSAVGLALWLDTEQQLDAVTAVSGSGPAYFF
LLIEAMTAAGEKLGLTRETAAQLTLQTALGAAHMAVSSDVDAAELRRRVTSPAGTTEAAI
KSFQAGGFEALVEKALGAAAHRSAEMAEQLGQ