Protein Info for AO356_12795 in Pseudomonas fluorescens FW300-N2C3

Annotation: heme biosynthesis operon protein HemX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 transmembrane" amino acids 33 to 54 (22 residues), see Phobius details PF04375: HemX" amino acids 129 to 357 (229 residues), 230.9 bits, see alignment E=8.6e-73

Best Hits

KEGG orthology group: K02496, uroporphyrin-III C-methyltransferase [EC: 2.1.1.107] (inferred from 85% identity to pfo:Pfl01_5485)

Predicted SEED Role

"Uroporphyrinogen-III synthase (EC 4.2.1.75)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.2.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 2.1.1.107 or 4.2.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAQ1 at UniProt or InterPro

Protein Sequence (377 amino acids)

>AO356_12795 heme biosynthesis operon protein HemX (Pseudomonas fluorescens FW300-N2C3)
VSETALPKEDLDQPAIDAPVEPPAPVAPRRGNGLAIVALLLGAAGVAVGGWGVWQVRHLQ
TNNQQQSAQVQALNDQAQALKLNEQRLSERLAQLPPAEELEERRRQVAQLQGDQQHLSQR
LETVLGASRKDWRLAEAEHLLRLASLRLSALQDIDSAQALVQGADEILREQNDPGSFAAR
EQLAKTLAALRSTEQPDRTGLFLQLGALRDQVLQLTELAPEYKDRGESLLGLTADGDGAS
RWAQWWDQISRYIRIDFNADENVRPLLAGQSLVQVRLALSLALEQAQWAALNGQAPVYTQ
ALTEARDVLKNNFNQDNPQSKIMLERVGELSKQPVTVVTPDLTGTLSSVQAYLERRNLNA
QESVKPLPAPGAQEATP