Protein Info for AO356_12620 in Pseudomonas fluorescens FW300-N2C3

Annotation: amino acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF01266: DAO" amino acids 2 to 398 (397 residues), 272.7 bits, see alignment E=1.6e-84 PF13450: NAD_binding_8" amino acids 5 to 40 (36 residues), 25.8 bits, see alignment 2.6e-09

Best Hits

Swiss-Prot: 92% identical to DADA_PSE14: D-amino acid dehydrogenase (dadA) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 99% identity to pba:PSEBR_a5508)

MetaCyc: 63% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H3B1 at UniProt or InterPro

Protein Sequence (434 amino acids)

>AO356_12620 amino acid dehydrogenase (Pseudomonas fluorescens FW300-N2C3)
MRVLVLGSGVIGTVSAYYLARAGFEVVVVDRQPAPAMETSFANAGQVSPGYASPWAAPGV
PLKAIKWLLQRHAPLAIKATADIDQYLWMAQMLRNCTASRYAINKERMVRLSEYSRDCLD
ELRAETGIAYEGRSLGTTQLFRTQAQLDGAAKDIAVLKESGVPFEVLDREGIARVEPALA
NVTDILAGALRLPNDQTGDCQMFTTRLAEMAVKLGVQFRFGQDIQRLDFAGDRINGVWID
GKLETADRYVLALGSYSPQLLKPLGIRAPVYPLKGYSLTVPITNPAMAPTSTILDETYKV
AITRFDNRIRVGGMAEIAGFDLSLNPRRRETLEMIVNDLYPQGGDLAEASFWTGLRPTTP
DGTPIVGATPFKNLFLNTGHGTLGWTMACGSGRLLADLMAKKKPQISAEGLDISRYGSKT
QESAKHVNPAPAHQ