Protein Info for AO356_12280 in Pseudomonas fluorescens FW300-N2C3

Annotation: EvpB family type VI secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 TIGR03355: type VI secretion protein, EvpB/VC_A0108 family" amino acids 19 to 493 (475 residues), 726 bits, see alignment E=1.1e-222 PF05943: VipB" amino acids 69 to 371 (303 residues), 453.3 bits, see alignment E=3.5e-140 PF18945: VipB_2" amino acids 381 to 492 (112 residues), 146.7 bits, see alignment E=3.1e-47

Best Hits

KEGG orthology group: K11900, type VI secretion system protein ImpC (inferred from 99% identity to pba:PSEBR_a5559)

Predicted SEED Role

"Uncharacterized protein ImpC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W7T7 at UniProt or InterPro

Protein Sequence (498 amino acids)

>AO356_12280 EvpB family type VI secretion protein (Pseudomonas fluorescens FW300-N2C3)
MTDNTAREGAQNLGATEETSEFASLLLQEFKPKTERAREAVETAVRTLAEQALAQTDLVS
NDAIKSIESIIAAIDAKLTAQVNQVIHHPDFQQLESAWRGLHYLVNNTETDEQLKIRVLN
ISKTDLHKTLKKFKGTAWDQSPLFKKMYEEEYGQFGGEPYGCLVGDYYFDQSPPDVELLG
ELSKVCAAMHSPFIAAASPTVMGMGSWQELSNPRDLTKIFTTPEYAGWRSLRESEDSRYI
GLTMPRFLARLPYGAKTDPVEAFAFEENTDGADSSKYTWANAAYAMAVNINRSFKHFGWC
SRIRGVESGGEVENLPAHTFPTDDGGVDMKCPTEIAISDRREAELAKNGFMPLLHKKNTD
FAAFIGAQSLQKPAEYDDPDATANANLAARLPYLFATCRFAHYLKCIVRDKIGSFKEKDE
MQRWLQDWILNYVDGDPAHSTETTKAQHPLAAAEVIVEEVEGNPGYYNSKFYLRPHYQLE
GLTVSLRLVSKLPSAKGA