Protein Info for AO356_11665 in Pseudomonas fluorescens FW300-N2C3

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 632 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 622 (616 residues), 912.6 bits, see alignment E=5.5e-279 PF01134: GIDA" amino acids 8 to 401 (394 residues), 573 bits, see alignment E=8.8e-176 PF12831: FAD_oxidored" amino acids 8 to 142 (135 residues), 34.2 bits, see alignment E=4.8e-12 PF21680: GIDA_C_1st" amino acids 462 to 556 (95 residues), 92.6 bits, see alignment E=5.4e-30 PF13932: GIDA_C" amino acids 561 to 616 (56 residues), 83 bits, see alignment 2.7e-27

Best Hits

Swiss-Prot: 98% identical to MNMG_PSEPF: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 98% identity to pba:PSEBR_a5661)

MetaCyc: 69% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WHA2 at UniProt or InterPro

Protein Sequence (632 amino acids)

>AO356_11665 tRNA uridine 5-carboxymethylaminomethyl modification protein (Pseudomonas fluorescens FW300-N2C3)
VDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLV
KEIDALGGAMAMATDKGGIQFRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWI
FQQAADDLIVEQDQVRGVVTQMGLRFFADSVVLTTGTFLGGLIHIGLQNFSGGRAGDPPS
IALAHRLRELPLRVGRLKTGTPPRIDGRSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIE
PEGLTTHELYPNGISTSLPFDVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLE
TKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAQGKDSWCPRRDEAYIGVLVDDLI
TLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDVRWAAFCTKRERIELEEQ
RLKSTWVRPGTEQGDAIAAKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGQGAEDPQVAE
QVEIKTKYAGYIDRQQDEIARLRASEDTKLPVDIDYTGISGLSKEIQSKLGATRPETLGQ
ASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA