Protein Info for AO356_09240 in Pseudomonas fluorescens FW300-N2C3

Annotation: hydroxyproline-2-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05544: Pro_racemase
  • amino acids 7 to 330 (324 residues), 420.9 bits, E=1.7e-130

Best Hits

Swiss-Prot: 75% identical to 4HPE2_AGRRK: 4-hydroxyproline 2-epimerase 2 (Arad_8151) from Agrobacterium radiobacter (strain K84 / ATCC BAA-868)

KEGG orthology group: None (inferred from 83% identity to pba:PSEBR_a3615)

Predicted SEED Role

"4-hydroxyproline epimerase (EC 5.1.1.8)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 5.1.1.8)

MetaCyc Pathways

• trans-4-hydroxy-L-proline degradation II (4/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.1.8

Use Curated BLAST to search for 5.1.1.8

Sequence Analysis Tools

See A0A0N9WVQ3 at UniProt or InterPro

Protein Sequence (332 amino acids)

>AO356_09240 hydroxyproline-2-epimerase (Pseudomonas fluorescens FW300-N2C3)
MRRSFFCIDSHACGNPVRVVAGGGPLLPSVSMAERREIFVRDHDWVRTALMFEPRGHDIM
SGVIIYPSTREDCDFGALFIEVSGCLPMCGAGTIGLSTVVIEEGLVTPKEPGKLAIETPA
GRVDVEYTLDGEYVESIKLFNVASYLHSADVVVQVPGVGQLTVDIAYGGNFYAVVEPQQC
WPGLDGHSTADIVGLSVKLRAALAGICNPLHPENERITGVHHVIWCDKPQNSTADGRCAV
FYGDKAIDRSPGGTGTSARMAQLFGKGRLKVGDTYRHESPIGTLYEGKVEAAIKVGEFDG
IRPSITGWAQVTGYNTLFVDDRDPLAHGFQLA