Protein Info for AO356_08065 in Pseudomonas fluorescens FW300-N2C3

Annotation: copper-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 797 transmembrane" amino acids 156 to 174 (19 residues), see Phobius details amino acids 186 to 206 (21 residues), see Phobius details amino acids 219 to 239 (21 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 403 to 422 (20 residues), see Phobius details amino acids 429 to 453 (25 residues), see Phobius details amino acids 743 to 762 (20 residues), see Phobius details amino acids 768 to 788 (21 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 8 to 68 (61 residues), 34.1 bits, see alignment 6.3e-12 amino acids 74 to 135 (62 residues), 48.5 bits, see alignment 2e-16 PF00403: HMA" amino acids 11 to 66 (56 residues), 48.6 bits, see alignment 1.3e-16 amino acids 75 to 134 (60 residues), 53.1 bits, see alignment 5.1e-18 TIGR01511: copper-translocating P-type ATPase" amino acids 200 to 786 (587 residues), 590.8 bits, see alignment E=7.9e-181 TIGR01525: heavy metal translocating P-type ATPase" amino acids 218 to 785 (568 residues), 630.9 bits, see alignment E=5.8e-193 TIGR01494: HAD ATPase, P-type, family IC" amino acids 256 to 763 (508 residues), 285.2 bits, see alignment E=2e-88 PF00122: E1-E2_ATPase" amino acids 284 to 384 (101 residues), 87.8 bits, see alignment E=5.8e-29 PF00702: Hydrolase" amino acids 480 to 697 (218 residues), 113.6 bits, see alignment E=2.5e-36

Best Hits

Swiss-Prot: 47% identical to COPA_STAHJ: Copper-exporting P-type ATPase (copA) from Staphylococcus haemolyticus (strain JCSC1435)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 96% identity to pba:PSEBR_a657)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WWC1 at UniProt or InterPro

Protein Sequence (797 amino acids)

>AO356_08065 copper-transporting ATPase (Pseudomonas fluorescens FW300-N2C3)
MSESTTFDLPIAGMTCASCAGRVERALSKVAGASAVSVNLATELARVQAPEGSLPALKQA
VEQAGYSVPVHTLELNIEGMTCASCVGRVERALGKVDGVNSVSVNLANERAHLELLGQVD
AQTLIDALKRAGYDATVWQAEQVADTQNNRLNRERLALVLAILLSVPLVLPMVLQPFGVH
WMLPAWVQFALATPVQFIFGARFYVAAFKAVRAGAGNMDLLVALGTSAGYGLSLYEWAIA
RPGSMPHLYFEASAVVIALVLLGKYLESRAKHQTASAIRALEALRPERAIQVIDGQERDV
AISALRLNDLVLVKPGERFPVDGEVLEGQSHADEALISGESLPVPKQPGDKVTGGAINGE
GRLLVRTQALGTETVLARIIRLVEDAQAAKAPIQKLVDKVSQVFVPVVLVLALATLIGWW
LYGAPLETALINAVAVLVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALERAHEVT
TVVFDKTGTLTSGTPRIAHLTALDGDEDALLKMAGALQRGSEHPLAKAVLDACTERGLDV
PDVSDSQSLAGRGIAGSLAGQRLALGNRRLLDELGLNPGTLAESAQAWEAEGRTLSWLLE
QGATPRVLGLFAFGDTLKPGALAAVQALNEQHIASHLLTGDNQGSARVVAQALEISNVHA
EVLPADKSAIVQQLKRHSVVAMVGDGINDAPALAAADIGIAMGGGTDVAMHAAGITLMRG
DPRLVPAALDISRKTYAKIRQNLFWAFVYNLIGIPLAAFGLLNPVLAGAAMALSSVSVVS
NALLLKFWTPKQLEDKR