Protein Info for AO356_07550 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): L-lactate and D-lactate permease (lctP family)
Rationale: This gene is often annotated as a L-lactate transporter but has a specific phenotype on D-lactate as well. Although it is difficult to rule out polar effects on the lactate dehydrogenase genes downstream, this gene's phenotype on D-lactate is conserved and is observed on both strands, and no other D-lactate transporter was identified by the mutant fitness data. (There is a zinc ABC transporter with a specific defect on D-lactate, but it is very similar to znuABC of P. aeruginosa, i.e. AO356_11480 is 65% identical to PA5498, so it is unlikely to be a lactate transporter.) Furthermore, the annotation as lactate permease seems to be derived from E. coli glcA or yghK, which transports both isomers of lactate (PMID:11283302). (KEGG_correct)
Original annotation: L-lactate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details amino acids 41 to 61 (21 residues), see Phobius details amino acids 72 to 94 (23 residues), see Phobius details amino acids 116 to 149 (34 residues), see Phobius details amino acids 154 to 180 (27 residues), see Phobius details amino acids 199 to 217 (19 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 252 to 269 (18 residues), see Phobius details amino acids 278 to 293 (16 residues), see Phobius details amino acids 311 to 330 (20 residues), see Phobius details amino acids 335 to 354 (20 residues), see Phobius details amino acids 360 to 360 (1 residues), see Phobius details amino acids 381 to 401 (21 residues), see Phobius details amino acids 419 to 437 (19 residues), see Phobius details amino acids 444 to 468 (25 residues), see Phobius details amino acids 540 to 559 (20 residues), see Phobius details TIGR00795: transporter, lactate permease (LctP) family" amino acids 7 to 556 (550 residues), 742.7 bits, see alignment E=1.3e-227 PF02652: Lactate_perm" amino acids 16 to 552 (537 residues), 696 bits, see alignment E=1.8e-213

Best Hits

KEGG orthology group: K03303, lactate transporter, LctP family (inferred from 100% identity to pba:PSEBR_a754)

Predicted SEED Role

"L-lactate permease" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WGP9 at UniProt or InterPro

Protein Sequence (564 amino acids)

>AO356_07550 L-lactate and D-lactate permease (lctP family) (Pseudomonas fluorescens FW300-N2C3)
MQTWQQLYSPLGSLGLSALAAVIPIVFFFLALAVFRLKGHVAGSITLALAIAVAIFAFNM
PADMAFAAAGYGFAYGLWPIAWIIVAAVFLYKLTVKSGQFEVIRSSVLSITDDQRLQVLL
IGFCFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGALGIPIIVA
GQVTGIDAFKIGAMTGRQLPLLSLFVPFWLVFMMDGLRGVRETWPAALVAGLSFAITQYF
TSNFIGPELPDITSALASLISLTLFLKVWQPKRTAGAQIAGATSSATVTASVGGFGQPRS
TVASPYSLGEIIKAWSPFLILTVLVTIWTLKPFKAMFAAGGSMYGWVFNFAIPHLDQMVI
KVAPIVINPTAIPAVFKLDPISATGTAIFFSALISMLVLKINIKTGLTTFKETLFELRWP
ILSIGMVLAFAFVTNYSGMSSTMALVLAGTGAAFPFFSPFLGWLGVFLTGSDTSSNALFS
SLQATTAHQIGVNDTLLVAANTSGGVTGKMISPQSIAVACAATGLVGKESDLFRFTLKHS
LFFATIVGLITLAQAYWFTGMLVH