Protein Info for AO356_07325 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): fructose phosphotransferase system, IIB/IIC components (EC 2.7.1.202)
Rationale: Specific phenotype: utilization of D-Fructose
Original annotation: PTS fructose transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 244 to 270 (27 residues), see Phobius details amino acids 282 to 308 (27 residues), see Phobius details amino acids 317 to 343 (27 residues), see Phobius details amino acids 363 to 383 (21 residues), see Phobius details amino acids 402 to 422 (21 residues), see Phobius details amino acids 441 to 465 (25 residues), see Phobius details amino acids 477 to 497 (21 residues), see Phobius details amino acids 503 to 523 (21 residues), see Phobius details amino acids 544 to 565 (22 residues), see Phobius details PF02302: PTS_IIB" amino acids 5 to 94 (90 residues), 45.9 bits, see alignment E=7.5e-16 amino acids 125 to 214 (90 residues), 78 bits, see alignment E=7.2e-26 TIGR00829: PTS system, Fru family, IIB component" amino acids 124 to 207 (84 residues), 111.8 bits, see alignment E=1.5e-36 TIGR01427: PTS system, Fru family, IIC component" amino acids 233 to 566 (334 residues), 477.5 bits, see alignment E=2.8e-147 PF02378: PTS_EIIC" amino acids 247 to 510 (264 residues), 80.5 bits, see alignment E=1.2e-26

Best Hits

Swiss-Prot: 66% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 94% identity to pba:PSEBR_a793)

MetaCyc: 66% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.202 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X6S6 at UniProt or InterPro

Protein Sequence (580 amino acids)

>AO356_07325 fructose phosphotransferase system, IIB/IIC components (EC 2.7.1.202) (Pseudomonas fluorescens FW300-N2C3)
MKLAIVTACPNGMVTSVLCARLLDAAAQRQGWSTSVEVHDAAHPERQLSAATLEAAEWVL
LVASGPVDLSRFVGKRVFRSTPALALQDVDAVLRRGAVEAEVLGEADASAQVPAASAERA
PRLVAITACPTGVAHTFMAAEALQQAATKLGYDLQVETQGSVGARNPLSAEAIAEADVVL
LATDIEVATERFAGKKIYRCGTGIALKQAEATLNKALVEARQESASSGASAPAKSEKTGA
YKHLLTGVSFMLPMVVAGGLMIALSFVFGIEAFKEPGTLAAALMQIGGETAFKLMVPLLA
GYIAYSIADRPGLAPGMIGGMLASTLGAGFIGGIIAGFLAGYAAKAISRYARLPQSLEAL
KPILIIPLLASLFTGLVMIYIVGKPVAGMLEALTHFLDSMGTTNAILLGVLLGAMMCVDL
GGPINKAAYAFSVGLLASQSYAPMAAAMAAGMVPPIGLGIATFIARRKFAQTEREAGKAA
LVLGLCFISEGAIPFAAKDPLRVIPASIAGGALTGALSMYFGCKLMAPHGGLFVMLIPNA
INHALLYLLAIVAGSLLTAVAYALLKRPEVVEMALEPAKA