Protein Info for AO356_05325 in Pseudomonas fluorescens FW300-N2C3

Annotation: branched-chain amino acid transporter permease subunit LivH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 45 to 64 (20 residues), see Phobius details amino acids 70 to 89 (20 residues), see Phobius details amino acids 102 to 122 (21 residues), see Phobius details amino acids 153 to 174 (22 residues), see Phobius details amino acids 202 to 224 (23 residues), see Phobius details amino acids 242 to 269 (28 residues), see Phobius details amino acids 281 to 299 (19 residues), see Phobius details PF02653: BPD_transp_2" amino acids 11 to 291 (281 residues), 180.1 bits, see alignment E=2.5e-57

Best Hits

Swiss-Prot: 84% identical to BRAD_PSEAE: High-affinity branched-chain amino acid transport system permease protein BraD (braD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01997, branched-chain amino acid transport system permease protein (inferred from 100% identity to pba:PSEBR_a1186)

MetaCyc: 67% identical to branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H1L0 at UniProt or InterPro

Protein Sequence (307 amino acids)

>AO356_05325 branched-chain amino acid transporter permease subunit LivH (Pseudomonas fluorescens FW300-N2C3)
MPDIYHFFQQLVNGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGL
SMMGLDSVPLLMTAAFLASIVVTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNT
VLLSQDSKDKSIPNLIPGNFAIGPGGAHEVLISYMQIVVFVVTLVAMLGLTLFISRSRLG
RACRACAEDIKMANLLGINTNNIIALTFVIGAALAAIAAVLLSMQYGVINPNAGFLVGLK
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILG
RPEVEKV