Protein Info for AO356_05200 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): D-mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for: D-Mannose. Often annotated as N-acylglucosamine 2-epimerase, but this is also distantly related to the D-mannose isomerase Sama_0560. The putative fructokinase AO356_00035 is also important for consuming mannose, which would complete the pathway.
Original annotation: sugar isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07221: GlcNAc_2-epim
  • amino acids 40 to 394 (355 residues), 415.4 bits, E=1e-128

Best Hits

Swiss-Prot: 46% identical to SQUS_SALTY: Sulfoquinovose isomerase (yihS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a1212)

MetaCyc: 46% identical to sulfoquinovose isomerase (Escherichia coli K-12 substr. MG1655)
Mannose isomerase. [EC: 5.3.1.7]; RXN-15296 [EC: 5.3.1.7, 5.3.1.31]

Predicted SEED Role

"N-acylglucosamine 2-epimerase (EC 5.1.3.8)" in subsystem Sialic Acid Metabolism (EC 5.1.3.8)

MetaCyc Pathways

• sulfoquinovose degradation III (2/4 steps found)
• N-acetylneuraminate and N-acetylmannosamine degradation II (1/3 steps found)
• sulfoquinovose degradation VI (2/5 steps found)
• sulfoquinovose degradation V (3/7 steps found)
• sulfoquinovose degradation I (1/5 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.8 or 5.3.1.31 or 5.3.1.7

Sequence Analysis Tools

See A0A0N9WVA7 at UniProt or InterPro

Protein Sequence (419 amino acids)

>AO356_05200 D-mannose isomerase (EC 5.3.1.7) (Pseudomonas fluorescens FW300-N2C3)
MDTFQPAFSSWLNAPAHQQWLAAEGLRLLAFAKASKLPDGFGNLDELGRLPADARAETMN
TARMTHSFAMAHIQGLPGFAELVDHGIQALNGRLRDAEHGGWFATTRPDEDGAGKAAYLH
AFVALAASSAVVAQRPGAAALLDEAVRIIDEHFWCEEEGALRESFNRDWSEEEAYRGANS
NMHATEAFLALADATDDPRWLVRALRIVERVIHGHAAANDYLVVEHFDRHWQPLHEYNQD
NPADGFRPYGTTPGHGFEWARLLLHLEAARVQIGMLTPGWLAQDAQKLFDQNCRHGWDVD
GAPGIVYTLDWDNRAVVRHRLHWVHAEAAAAASALLKRTDEAKYEAWYRCFWEFCDKHFI
DRCNGSWHHELDPQNRPSADIWPGKPDLYHAWQAVLIPRLPLAPSMASALARLSSPAPV