Protein Info for AO356_05195 in Pseudomonas fluorescens FW300-N2C3

Annotation: sugar ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details

Best Hits

Swiss-Prot: 44% identical to SP39_RHILO: Probable sugar-binding periplasmic protein (mlr3050) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K02027, multiple sugar transport system substrate-binding protein (inferred from 99% identity to pba:PSEBR_a1213)

Predicted SEED Role

"Glucose ABC transport system, periplasmic sugar-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W4R2 at UniProt or InterPro

Protein Sequence (433 amino acids)

>AO356_05195 sugar ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2C3)
MNAISRLATVISLASLLPVAAFPVTTLAAESKGSVEVVHWWTSGGEKAAVDVLKAQVEKD
GFTWKDGAVAGGGGSTAMTVLKSRAVAGNPPGVAQIKGPDIQEWASTGLLDTDILKDVAK
AEKWDSLLDKKVSDTVKYDGDYVAVPVNIHRVNWLWINPEVFKKAGITKNPTTLEEFYAA
GDKLKAAGFIPLAHGGQPWQDSTVFEAVVLSVMGADGYKKALVDLDNKALTGPEMVKALT
ELKKVATYMDADGKGQDWNLEAAKVINGKAGMQIMGDWAKSEWTAAKKVAGKDYECVAFP
GTDKAFTYNIDSLAVFKQKDAGTAAGQQDIAKVVLGENFQKVFSINKGSIPVRNDMLGDM
AKYGFDSCAQTAAKDFLTDAKSGGLQPSMAHNMATTLAVQGAFFDVVTNYINDPKADPAD
AAKKLGAAVQSAK