Protein Info for AO356_04960 in Pseudomonas fluorescens FW300-N2C3

Annotation: nuclease PIN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 52 to 80 (29 residues), see Phobius details amino acids 92 to 115 (24 residues), see Phobius details amino acids 121 to 141 (21 residues), see Phobius details amino acids 156 to 180 (25 residues), see Phobius details amino acids 204 to 223 (20 residues), see Phobius details amino acids 229 to 250 (22 residues), see Phobius details amino acids 371 to 392 (22 residues), see Phobius details amino acids 416 to 433 (18 residues), see Phobius details amino acids 463 to 485 (23 residues), see Phobius details PF01384: PHO4" amino acids 30 to 478 (449 residues), 310.3 bits, see alignment E=7.8e-97

Best Hits

Swiss-Prot: 53% identical to PITB_ECOLI: Probable low-affinity inorganic phosphate transporter 2 (pitB) from Escherichia coli (strain K12)

KEGG orthology group: K03306, inorganic phosphate transporter, PiT family (inferred from 95% identity to pfo:Pfl01_1262)

MetaCyc: 53% identical to metal phosphate:H+ symporter PitB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-114; TRANS-RXN-277; TRANS-RXN-278; TRANS-RXN0-550

Predicted SEED Role

"Low-affinity inorganic phosphate transporter" in subsystem Phosphate metabolism

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X5K9 at UniProt or InterPro

Protein Sequence (491 amino acids)

>AO356_04960 nuclease PIN (Pseudomonas fluorescens FW300-N2C3)
MIDLFSGLDAWVLVSLLLALAFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVF
NFLGVLLGGVGVAYAIVHLLPVELLINVNTGHGLAMVFSLLAAAITWNLGTWYFGIPASS
SHTLIGSILGVGLANALINDIPLADGVNWQKAIDIGASLVFSPMAGFLIAALVLIGLKWW
RPLSKMHKTPEQRRQIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPAQFVLDLGSTTYQIERTRDATLHLSQFYQRNNESLGEFLALGKSVEGDLPEKFRCNPQ
QTEPTINALLDTLKGVADYHSLPSERRIEVRRYLLCLDDTAKKVGKLPGLAAREKDDLNK
LRKDLTATTEYAPFWVILAVALALGLGTMIGWKRVVLTIGEKIGKQGMTYAQGMSAQITT
ACMIGAANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVRNILLAWVLTLPATVALSAG
LFWLASKALGS