Protein Info for AO356_04915 in Pseudomonas fluorescens FW300-N2C3

Annotation: alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 transmembrane" amino acids 29 to 50 (22 residues), see Phobius details amino acids 59 to 81 (23 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 119 to 137 (19 residues), see Phobius details amino acids 158 to 180 (23 residues), see Phobius details amino acids 192 to 211 (20 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 280 to 299 (20 residues), see Phobius details amino acids 310 to 328 (19 residues), see Phobius details amino acids 335 to 358 (24 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 403 to 421 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 22 to 218 (197 residues), 96.1 bits, see alignment E=3.5e-31 amino acids 220 to 417 (198 residues), 40.3 bits, see alignment E=3e-14 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 27 to 416 (390 residues), 469.6 bits, see alignment E=4.6e-145 PF07690: MFS_1" amino acids 55 to 381 (327 residues), 102.3 bits, see alignment E=4.2e-33 amino acids 271 to 425 (155 residues), 40.6 bits, see alignment E=2.3e-14 PF12832: MFS_1_like" amino acids 260 to 417 (158 residues), 28.5 bits, see alignment E=1.2e-10

Best Hits

Swiss-Prot: 70% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 98% identity to pba:PSEBR_a1270)

MetaCyc: 70% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WV37 at UniProt or InterPro

Protein Sequence (432 amino acids)

>AO356_04915 alpha-ketoglutarate permease (Pseudomonas fluorescens FW300-N2C3)
MATPIISHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDNPTVQLV
NTAGVFAAGFLMRPIGGWIFGRVADRHGRKNSMMISVLMMCFGSLLIACLPTYKDIGVWA
PVLLLFARLLQGLSVGGEYGTTATYMSEVALKGQRGFFASFQYVTLIGGQLLAVSLVVIL
QQFLNEDELRAYGWRIPFVVGAVAALISLFLRRSLKETSSKEMRENKDAGSIAALFRDHK
AAFVTVLGYTAGGSLIFYTFTTYMQKYLVNTAGLHAKTASYIMTGALFLYMCMQPLFGML
ADKIGRRNSMLWFGGLGALCTVPILLTLKSISSPFLAFILITLALAIVSFYTSISGLVKA
EMFPPEVRALGVGLAYAVANAIFGGSAEYVALSLKAQGMENAFYWYVTVMMVVAFLFSLR
LPKQPAYLHHDL