Protein Info for AO356_03400 in Pseudomonas fluorescens FW300-N2C3

Annotation: aminotransferase DegT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 PF01041: DegT_DnrJ_EryC1" amino acids 14 to 358 (345 residues), 405.7 bits, see alignment E=5.1e-125 PF01212: Beta_elim_lyase" amino acids 28 to 269 (242 residues), 38.7 bits, see alignment E=1.9e-13 PF01053: Cys_Met_Meta_PP" amino acids 35 to 159 (125 residues), 29.2 bits, see alignment E=9e-11 PF00266: Aminotran_5" amino acids 36 to 162 (127 residues), 29.2 bits, see alignment E=1.2e-10 PF00155: Aminotran_1_2" amino acids 37 to 214 (178 residues), 49.8 bits, see alignment E=7.5e-17

Best Hits

Swiss-Prot: 81% identical to WBPE_PSEAE: UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase (wbpE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K13017, UDP-3-keto-D-GlcNAcA aminotransferase [EC: 2.6.1.-] (inferred from 79% identity to acd:AOLE_19120)

MetaCyc: 81% identical to UDP-2-acetamido-2-deoxy-ribo-hexuluronate aminotransferase (Pseudomonas aeruginosa PAO1)
RXN-13596 [EC: 2.6.1.98]

Predicted SEED Role

"Glutamate--UDP-2-acetamido-2-deoxy-D-ribohex-3-uluronic acid aminotransferase (PLP cofactor)"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.-

Use Curated BLAST to search for 2.6.1.- or 2.6.1.98

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X4Q1 at UniProt or InterPro

Protein Sequence (360 amino acids)

>AO356_03400 aminotransferase DegT (Pseudomonas fluorescens FW300-N2C3)
MIEFIDLKSQQARIKDKIDAGIQRVLSHGQYILGPEVAELEEKLAQFVGAKHCITCANGT
DALQIAQMALGIGPGDEVITPGFTYIATAETVALLGAKPVYVDIDPRTYNLDPQKLEAAI
TPRTKAIIPVSLYGQCADYDAINAIAVKYSIPVIEDAAQSFGATYKGKRSCNLTTIACTS
FFPSKPLGCYGDGGAIFTNDDELAKVLRQIARHGQDRRYHHVRVGVNSRLDTLQAAILLP
KLEIFEKELEQRQRVARLYGESLEALGVKTIPYVEEWNTSAYAQYTILVDERDQLQDKLK
KQGIPTAVHYPIPLNKQPAVADANAHLPTGDRVAEQVMSLPMHPYLGDEAVAIICAAVAG