Protein Info for AO356_01595 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4)
Rationale: Specifically important for utilizing L-Leucine.
Original annotation: 3-methylcrotonyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details PF00289: Biotin_carb_N" amino acids 7 to 114 (108 residues), 143.1 bits, see alignment E=1.6e-45 PF02786: CPSase_L_D2" amino acids 120 to 327 (208 residues), 269.5 bits, see alignment E=6.4e-84 PF02222: ATP-grasp" amino acids 141 to 296 (156 residues), 39.7 bits, see alignment E=1.4e-13 PF07478: Dala_Dala_lig_C" amino acids 144 to 295 (152 residues), 47.7 bits, see alignment E=4.8e-16 PF02785: Biotin_carb_C" amino acids 340 to 447 (108 residues), 126.9 bits, see alignment E=1.2e-40 PF21139: MCC_alpha_BT" amino acids 462 to 563 (102 residues), 112.2 bits, see alignment E=4.4e-36 PF00364: Biotin_lipoyl" amino acids 584 to 646 (63 residues), 61.3 bits, see alignment E=2.2e-20

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 96% identity to pba:PSEBR_a1842)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VXF3 at UniProt or InterPro

Protein Sequence (649 amino acids)

>AO356_01595 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (Pseudomonas fluorescens FW300-N2C3)
MSAPALTTLLVANRGEIACRVMRTARAMGLTTVAVHSATDRDARHSREADIRVDLGGSKA
ADSYLQIDKLIAAAKASGAQAIHPGYGFLSENAGFARAIENAGLIFLGPPASAIDAMGSK
SAAKTLMETAGVPLVPGYHGEAQDLETFRDAAERIGYPVLLKATAGGGGKGMKVVEDVSQ
LAEALASAQREAQSSFGDSRMLVEKYLLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQ
KVVEEAPAPGLTPQLRRAMGEAAVRAAQAIGYVGAGTVEFLLDARGEFFFMEMNTRLQVE
HPVTEAITGLDLVAWQIRVAQGEPLPITQAQVPLLGHAIEVRLYAEDPGNDFLPATGRLA
LYRESAEGPGRRVDSGVEEGDEISPFYDPMLGKLIAWGEDREQARLRLLSMLDEFVIGGL
KTNIGFLRRIVAHPAFAAAELDTGFIPRYQAQLLPEPSELDDAFWFAAAQGVALSLAPHV
RGDDAGSPWASTTGMRLGLPRETTLHLSCEGQDRALTLDVTAHCAELEGERLTIEHHGVR
RSHRAIRQVDSLYLQWEGDLHRIDLYDPLAAAEASHSHQGGLVAPMNGSIVRVLVGVGQT
VEAGAQLVVLEAMKMEHSIRAPKAGVIKALYCQEGEMVSEGSALVAFEE