Protein Info for AO356_01240 in Pseudomonas fluorescens FW300-N2C3
Annotation: glucarate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida
KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 99% identity to pba:PSEBR_a1901)MetaCyc: 82% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]
Predicted SEED Role
"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)
MetaCyc Pathways
- D-glucarate degradation II (3/3 steps found)
- superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
- D-glucarate degradation I (3/4 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (17/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.40
Use Curated BLAST to search for 4.2.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N7H1B3 at UniProt or InterPro
Protein Sequence (447 amino acids)
>AO356_01240 glucarate dehydratase (Pseudomonas fluorescens FW300-N2C3) MSIPETSKAPIITSMQVVPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGG ERIRQTLEDARSLVVGSPIGTYQKILNTVRNAFADRDAGGRGLQTFDLRITIHAVTGLEA ALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRQQTDLAYRSEPDADNDWFRVR HEKALDADAVVRLAEAAQARYGFQDFKLKGGVLQGDAEIEAVTALAERFPQARITLDPNG AWSLKEAIRLCRDQHKVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREM GHAITLQSVDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAA PGDITAIDTHWIWQDGQRLTKAPLQIQGGCVQVPKKPGLGIELDTDQLAKAHELYKGMGL GARDDAVAMQFLIPGWTFNNKRPCLVR