Protein Info for AO356_00950 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): Ribokinase (EC 2.7.1.15)
Rationale: Specifically important for utilizing D-Ribose. Automated validation from mutant phenotype: the predicted function (RIBOKIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: ribokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 PF00294: PfkB" amino acids 3 to 295 (293 residues), 242.5 bits, see alignment E=6.8e-76 TIGR02152: ribokinase" amino acids 5 to 300 (296 residues), 369.4 bits, see alignment E=6.9e-115 PF08543: Phos_pyr_kin" amino acids 180 to 277 (98 residues), 38.5 bits, see alignment E=8.8e-14

Best Hits

Swiss-Prot: 44% identical to RBSK_SCHPO: Ribokinase (rbk1) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K00852, ribokinase [EC: 2.7.1.15] (inferred from 94% identity to pba:PSEBR_a3707)

MetaCyc: 43% identical to ribokinase (Escherichia coli K-12 substr. MG1655)
Ribokinase. [EC: 2.7.1.15]

Predicted SEED Role

"Ribokinase (EC 2.7.1.15)" in subsystem D-ribose utilization or Deoxyribose and Deoxynucleoside Catabolism (EC 2.7.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H1A6 at UniProt or InterPro

Protein Sequence (304 amino acids)

>AO356_00950 Ribokinase (EC 2.7.1.15) (Pseudomonas fluorescens FW300-N2C3)
MPAKVVVIGSLNMDLVTRAPRLPRGGETLIGESFATIPGGKGANQAVAAARLGAQVSMVG
CVGDDAYGEQLRGALLAEGIDCQAVRVEQGSSGVALIVVDDNSQNAIVIVAGANGALTAE
VLDGVDDVLQSADVIICQLEVPDATVGHALKRGRALGKIVILNPAPASHALPADWYACID
YLIPNESEAAVLSGLAVDSLETAEAAAAHLIAAGAGKVIVTLGAQGLMFANGASFEHFPA
PRVKAVDTTAAGDTFVGGFAAALACGKSEVDAIRFGQVAAALSVTRAGAQPSIPTLLEVQ
AFKS