Protein Info for AO356_00940 in Pseudomonas fluorescens FW300-N2C3
Updated annotation (from data): uridine/adenosine nucleosidase (EC 3.2.2.3; EC 3.2.2.7)
Rationale: Specifically important for: Uridine. Also important on adenosine. Annotated by SEED as acting on uridine and inosine, and the fitness data suggests that it might also act on inosine (subtle phenotype)
Original annotation: nucleoside hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a3705)Predicted SEED Role
"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)
MetaCyc Pathways
- superpathway of purines degradation in plants (15/18 steps found)
- purine nucleotides degradation I (plants) (10/12 steps found)
- adenosine nucleotides degradation I (7/8 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- adenine and adenosine salvage II (2/2 steps found)
- guanine and guanosine salvage II (2/2 steps found)
- pyrimidine ribonucleosides salvage III (2/2 steps found)
- guanosine nucleotides degradation I (3/4 steps found)
- superpathway of pyrimidine ribonucleosides salvage (7/10 steps found)
- pyrimidine ribonucleosides salvage II (1/2 steps found)
- NAD salvage (plants) (6/11 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.2.2.1 or 3.2.2.3 or 3.2.2.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WKN2 at UniProt or InterPro
Protein Sequence (342 amino acids)
>AO356_00940 uridine/adenosine nucleosidase (EC 3.2.2.3; EC 3.2.2.7) (Pseudomonas fluorescens FW300-N2C3) MHRYAEKMHQLIRSLLLLSLITATSAQAAEKIDLIIDTDPGADDVVALLFALASPEELHI RALTTVAGNVRLDKTSRNARLAREWAGREDVPVYAGAPKPLMRTPIYAENIHGKEGLSGV TVHEPKKGLAEGNAVNYLIDTLKAAKPHSITIAMLGPQTNLALALVQEPDIVQGIKEVVI MGGAHFNGGNITPVAEFNLFADPQAAEVVAKSSVKLTYLPLDVTHKILTSEARLKQIAAL NNNASKLVGDILNEYVKGDMEHYGMTGGPVHDATVIAYLLKPQLFTGRSVNVVVDSREGP TFGQTIVDWYDGLKAPKNAFWVENGDAQGFFDLLTERLARLK