Protein Info for AO356_00695 in Pseudomonas fluorescens FW300-N2C3
Annotation: multidrug transporter
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 68% identity to aav:Aave_2111)MetaCyc: 60% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WCJ8 at UniProt or InterPro
Protein Sequence (1048 amino acids)
>AO356_00695 multidrug transporter (Pseudomonas fluorescens FW300-N2C3) MARFFIDRPIFAWVIAIVIMLAGALSISQLPLEQYPDIAPPTVRISATYTGASAKTVEDS VTQVIEQQMKGLDNLTYMSASSSSDGSASISLTFTAGTNPDVAQMQVQNKLQQAESRLPQ SVQSEGLTVTKGGSDFLMIAALASDNPGVTGTQIGDYISSTLLDSISRIDGVGDVQTLGS GYAMRIWLDPALLEKYALMPSDVSTALEAQNTEVSAGQLGAMPAVKGQQLNATISARSKL QTVEEFRNVVVKSTSDGAVVLLGDVARVELGSESYDVSSALNGKPAAAMGVQLAAGANAL NVGEAVKAKLKELEAFYPTELQLKNVIAYDTTPFVSLSIEEVVKSLGEAIVLVVLIMFLF LQNLRATLIPAITVPVVLLGTFGVLALFGYSINTLTMFAMVLAIGLLVDDAIVVVENVER VMGEQGLSALDATRQSMDEITSALVGIALVLSAVFIPMAFFGGSTGVIYRQFSVTIVSAM VLSVLVAMTLTPALCATLLKPSDSQGHGPQRGFFGWFNRTFERAAEAYKRQVGGILQRGR VGWLVYGLVLLAMAAGYFSLPTSFLPDEDQGILMAQVQLPVGATDSRTQAVVKQFESYLL EQPEVEALISISGLGMNGNSQNSARAFIRLKDWSERTGAGQDAASFAQRATMALSSIGDA NVFVMQPPAVRGLGQTSGFDLQLKDLAGLGHDALVAAREKLIGQANQDPRLLGVRSNGLD DAPQLKVNIDDRKAGALGLSTSDINTTLSTALGGTYVNDFLNQGRVKKVYVQGEAAARMQ AADLEHWFVRNSNDEMVPFSSFASSSWSYGSPLLERYNGNASMEVVGDPAPGISSGDAMD AVEALIKQLPEGIGYEWTGQSYQLRLSGSQAPLLYGISVLFVFLCLAALYESWSVPFSVM LVVPLGVVGAVLATRVSGLSNDVYFQVGLLTTVGLAAKNAILIVEFAKHLQEQGNSLREA TLIAVRQRLRPILMTSLAFMFGVLPLALSSGAGSAGRQAIGTGVLGGMLSATVLGIFFVP LFFVQIRRRFARVSTASTSVPSAQSGDA