Protein Info for AO356_00010 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): ABC transporter for D-Sorbitol, ATPase component
Rationale: Specific phenotype on D-Sorbitol.
Original annotation: ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 PF00005: ABC_tran" amino acids 20 to 161 (142 residues), 123 bits, see alignment E=2.2e-39 PF17912: OB_MalK" amino acids 235 to 288 (54 residues), 37.3 bits, see alignment 6.2e-13 PF08402: TOBE_2" amino acids 281 to 350 (70 residues), 32.6 bits, see alignment E=1e-11

Best Hits

Swiss-Prot: 54% identical to MALK_YERPS: Maltose/maltodextrin import ATP-binding protein MalK (malK) from Yersinia pseudotuberculosis serotype I (strain IP32953)

KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a2027)

MetaCyc: 73% identical to polyol ABC-type transporterATP-binding component MtlK (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, ATP-binding component" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WSQ8 at UniProt or InterPro

Protein Sequence (365 amino acids)

>AO356_00010 ABC transporter for D-Sorbitol, ATPase component (Pseudomonas fluorescens FW300-N2C3)
MATLKIENLKKGFEGLSIIKGIDLEVKDKEFVVFVGPSGCGKSTLLRLIAGLEDVTSGTI
ELDGRDITEVTPAKRDLAMVFQTYALYPHMTVRKNLSFALDLAGEKKPDVERKVAEAARI
LELGSLLDRKPKQLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQTRLELSRLH
KELQATMIYVTHDQVEAMTLATKVVVLNAGRIEQIGSPLELYHHPANLFVAGFLGTPKMG
FLQATVHAVHASGVEVRFASGTTLLIPRDSSALSVGQSVTIGIRPEHLTLGAEGQVLVTT
DVTERLGSDTFCHVNVDSGESLTVRVQGDCEVPYAARRYLTLDVAHCHLFDESGLSVSPA
ASRAA