Protein Info for AO353_29320 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): putrescine ABC transporter, permease component
Rationale: Specific phenotype: utilization of Putrescine Dihydrochloride
Original annotation: spermidine/putrescine ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 64 to 85 (22 residues), see Phobius details amino acids 97 to 124 (28 residues), see Phobius details amino acids 135 to 158 (24 residues), see Phobius details amino acids 179 to 202 (24 residues), see Phobius details amino acids 239 to 258 (20 residues), see Phobius details PF00528: BPD_transp_1" amino acids 75 to 253 (179 residues), 48 bits, see alignment E=6.4e-17

Best Hits

Swiss-Prot: 65% identical to POTI_ECOL6: Putrescine transport system permease protein PotI (potI) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11074, putrescine transport system permease protein (inferred from 93% identity to pfo:Pfl01_2118)

MetaCyc: 65% identical to putrescine ABC transporter membrane subunit PotI (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotI (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H149 at UniProt or InterPro

Protein Sequence (299 amino acids)

>AO353_29320 putrescine ABC transporter, permease component (Pseudomonas fluorescens FW300-N2E3)
MNRFGFSRMMLVLGLLFIYMPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSV
VRSLEIACYTSIAAVALGTLAAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
VAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGARPLKVF
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSIVTFMVWYFSRKAEATRKRAIQEAMDQTASESWKQPASQRPATQQIDATA