Protein Info for AO353_29300 in Pseudomonas fluorescens FW300-N2E3

Annotation: gamma-glutamylputrescine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 121 to 454 (334 residues), 360.2 bits, E=5.5e-112

Best Hits

Swiss-Prot: 45% identical to PUUA_ECOLI: Gamma-glutamylputrescine synthetase PuuA (puuA) from Escherichia coli (strain K12)

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 98% identity to pfo:Pfl01_2122)

MetaCyc: 45% identical to glutamate-putrescine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--putrescine ligase. [EC: 6.3.1.11]

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of ornithine degradation (7/8 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• putrescine degradation II (3/4 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.11, 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See A0A0N9VVV8 at UniProt or InterPro

Protein Sequence (458 amino acids)

>AO353_29300 gamma-glutamylputrescine synthetase (Pseudomonas fluorescens FW300-N2E3)
MSVPLRAVQLNEANAFLKKYPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
LFALDINGSTVESTGLGLDIGDSDRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFF
ADPREVLANVVRKFDEMGLTICAAFELEFYLIDQDNVNGRPQSPRSPVSGKRPMSTQVYL
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGV
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGIDNRTVAVRVPTGSADAVRIEHRVAG
ADANPYLLMASVLAGIHHGLTNEIEPGPPVEGNSYEQNEQSLPNNLRDALRELDDSEVMA
RYIDPLYIDVFVACKESELAEFENSISDLEYNWYLHTV