Protein Info for AO353_29295 in Pseudomonas fluorescens FW300-N2E3

Annotation: aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF00171: Aldedh" amino acids 34 to 491 (458 residues), 555.8 bits, see alignment E=6.5e-171 PF05893: LuxC" amino acids 150 to 325 (176 residues), 23.1 bits, see alignment E=3.3e-09

Best Hits

Swiss-Prot: 56% identical to PUUC_ECOLI: NADP/NAD-dependent aldehyde dehydrogenase PuuC (puuC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_2123)

MetaCyc: 77% identical to 4-guanidinobutyraldehyde dehydrogenase / 4-aminobutyraldehyde dehydrogenase (Pseudomonas aeruginosa)
Gamma-guanidinobutyraldehyde dehydrogenase. [EC: 1.2.1.54]; Aminobutyraldehyde dehydrogenase. [EC: 1.2.1.54, 1.2.1.19]

Predicted SEED Role

"Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-)" (EC 1.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.-, 1.2.1.19, 1.2.1.54

Use Curated BLAST to search for 1.2.1.- or 1.2.1.19 or 1.2.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WS10 at UniProt or InterPro

Protein Sequence (496 amino acids)

>AO353_29295 aldehyde dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MAITRSGWEQHFQSLTIEGRAFIDGHYCSALSRDTFECVSPVDGRFLANVASTDEADANA
AVQVARRAFDSGVWAKLAPAERKRILIRFADLILANQEELALLETLDMGKPISDSMAIDI
PATANAIRWSAEAIDKIYDEVAATPHDQLGLVTREPSGVVAAIVPWNFPLIMASWKFAPA
LAAGNSFILKPSEKSPLTAIRIAQLALDAGIPKGVFNVLPGFGHTVGKALALHMDVDVLA
FTGSTAIAKQLLIYAGQSNMKRVWLEAGGKSPNVVFADAPDLRAAATAAASAIAFNQGEV
CTAGSRLLVERSIREQFIPLLVEALQAWKPGHALDPETTVGAVVDQRQLDNVLRYISIGK
EQGAQLIAGGNRALEDTGGLYVEPAIFDGVTNAMTIAREEIFGPVLSLITFDTAEEALAI
ANDSIFGLAAGVWTSNLSKAHTFARGLRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSL
HAFDKYTELKATWIKL