Protein Info for AO353_29150 in Pseudomonas fluorescens FW300-N2E3
Annotation: amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01426, amidase [EC: 3.5.1.4] (inferred from 91% identity to pfo:Pfl01_3257)Predicted SEED Role
"N-carbamoylputrescine amidase (EC 3.5.1.53)" (EC 3.5.1.53)
MetaCyc Pathways
- L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) (3/3 steps found)
- putrescine biosynthesis II (3/3 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- superpathway of polyamine biosynthesis II (6/8 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (2/3 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- indole-3-acetate biosynthesis II (4/12 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4, 3.5.1.53
Use Curated BLAST to search for 3.5.1.4 or 3.5.1.53
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9W1C1 at UniProt or InterPro
Protein Sequence (568 amino acids)
>AO353_29150 amidase (Pseudomonas fluorescens FW300-N2E3) MIEVTEVPIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTPTALNAVVVRNPEALNEA RASDARRAKGQTLGPLDGIPYTAKDSYLVKGLTAASGSPAFANLIAYRDAFTIERLRAAG AICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFASGSSNGAGTATAASFAAFGLA EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPFARTMADLLEVLDVVVA EDPDTRGDLWRMQPWVPIPSVTSVRPASYVALAANPDALAGKRFGIPRMYINADPEAGTS EAPGIGGPTGQRINTRASVIGLWEQARQALEAAGAEVIDVDFPLVSNCEGDRPGAPTVFN RGIVSKEFLHDELWDLTAWAFDDFLQANGDPKLNRLVDVNGPLIFPHDPGTLPNREGDLA AGMDEYVRMAERGITPWDQISTVPDGLRGLEQTRKIDLEDWMDKLGLDAVLFPTVADVGP ANADVDPISADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDD STLLRLASAFEATGTKRLIPPRTPVLRK