Protein Info for AO353_28990 in Pseudomonas fluorescens FW300-N2E3

Annotation: LOG family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14793: DUF4478
  • amino acids 10 to 116 (107 residues), 144.8 bits, E=1.7e-46
• PF03641: Lysine_decarbox
  • amino acids 192 to 331 (140 residues), 94.1 bits, E=1.6e-30
• PF11892: PpnN_C
  • amino acids 336 to 455 (120 residues), 186.1 bits, E=4.6e-59

Best Hits

Swiss-Prot: 56% identical to PPNN_ECOLI: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli (strain K12)

KEGG orthology group: K06966, (no description) (inferred from 91% identity to pba:PSEBR_a3435)

MetaCyc: 56% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

• adenosine nucleotides degradation III (1/1 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

See A0A0N9W0S6 at UniProt or InterPro

Protein Sequence (457 amino acids)

>AO353_28990 LOG family protein (Pseudomonas fluorescens FW300-N2E3)
MTARQVINASVSPKGSLETLSQREVQQLSAAGSGSIYSLFRQCALAILNTGAHVDNAKTI
LEAYRDFEIRIHQQDRGVRLELLNAPADAFVDGEMIASTREMLFSALRDIVYTENELDSQ
RIDLSSSQGISDYVFHLLRNARTLRAGVEPKIVVCWGGHSISSEEYQYTKKVGHELGLRS
LDVCTGCGPGVMKGPMKGATISHAKQRITHGRYLGLTEPGIIAAEAPNPIVNELVILPDI
EKRLEAFVRVGHGIIIFPGGAGTAEEFLYLLGILMHPDNRDLPFPVILTGPKSAEPYLQQ
LHAFVGATLGKDAQSHYEIIIDDPAQVARQMTKGLKDVKQFRRERNDAFHFNWLLKIDEG
FQRPFDPTHENMASLQLRHDLPPHELAANLRRAFSGIVAGNVKDKGIRLIEKYGPYKIHG
DAAVMQPLDKLLKAFVAQHRMKLPGGAEYVPCYQVIS