Protein Info for AO353_28460 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 56 to 78 (23 residues), see Phobius details amino acids 85 to 105 (21 residues), see Phobius details amino acids 111 to 133 (23 residues), see Phobius details amino acids 144 to 166 (23 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 206 to 227 (22 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details amino acids 277 to 306 (30 residues), see Phobius details amino acids 314 to 335 (22 residues), see Phobius details amino acids 342 to 360 (19 residues), see Phobius details amino acids 372 to 395 (24 residues), see Phobius details amino acids 416 to 439 (24 residues), see Phobius details amino acids 496 to 518 (23 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 19 to 507 (489 residues), 230.8 bits, see alignment E=1.6e-72 PF07690: MFS_1" amino acids 22 to 421 (400 residues), 107.4 bits, see alignment E=3.9e-35

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 76% identity to sme:SM_b20698)

Predicted SEED Role

"FIG01074225: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WRP3 at UniProt or InterPro

Protein Sequence (528 amino acids)

>AO353_28460 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
MNTFASATPSGAKATTGVLLIVGVVLATLTEAIAGTVLSIGRGDIIGDTYATPDEIAWLD
VGYTTLKLIGFIMAAWLMQRFSARNLIIGSTLAMGLACCMAAIIARLDLLIALRIIQGFA
GGTLLVAGQAMIFRAWPRAFQPILQALFAMGSVVAPATIAPALQGWLIDSQSWTRIFFSV
VPLALAATGLLLIADGPSPAQVRQRPFDWLGFSLISATLFCFTYVLSQGSRWDWFEEPRI
LMLTMIGTAALLVFFGQQALAKGLGVFDFTVFKSSDFTFAFIVSFVAGAALFGSAFLIPA
FALSVLAFTPTDAGLLLLPSGGLFVGSLLITAFFIKVRRVPPVATVPFGILLIMGAMWML
SGSTSDSGAGDMMAAILLRGLGLGFLFLSITLIAFSDLNSRNLAFGIGLFNTGRQLGGLM
GVAGIQTLIDHHIVINVAALGANVTPGGNALIERLTATTAMLAGKGMDQVAAGRAATSLL
GKVVSGQATVIAFDTAFYAVALLFVVAAPILIGIKIGLVRYAKARAVA