Protein Info for AO353_28090 in Pseudomonas fluorescens FW300-N2E3
Annotation: dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 42% identical to A1A1B_DANRE: Aldo-keto reductase family 1 member A1-B (akr1a1b) from Danio rerio
KEGG orthology group: None (inferred from 70% identity to mno:Mnod_6439)MetaCyc: 42% identical to alcohol dehydrogenase [NADP+] (Mus musculus)
Glucuronate reductase. [EC: 1.1.1.19]
Predicted SEED Role
"2,5-diketo-D-gluconic acid reductase (EC 1.1.1.-)" (EC 1.1.1.-)
MetaCyc Pathways
- L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
- D-glucuronate degradation I (2/5 steps found)
- L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Polyketide sugar unit biosynthesis
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-
Use Curated BLAST to search for 1.1.1.- or 1.1.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WIX3 at UniProt or InterPro
Protein Sequence (316 amino acids)
>AO353_28090 dehydrogenase (Pseudomonas fluorescens FW300-N2E3) MSYEAFHDELRDTKFPLVHGSGAMPAVGFGTLFRDLSVTTQAIKHALETGFRHFDCAERY RNEDKVGVAIKEFLETGKVRREDLFITTKLWNTNHRPERVLPAFEASCRRLQVDYIDCYL IHTPFAFQPGEDQEPRDEQGRIIYDSGVTLIETWRALERLVDEGRCKSIGLSDITLQALQ EIVAVARIKPAVVQVESHPYLPEWELLEFCRQHGIIVLAFAPLGHGMQPNVLEDAVITGV ARRLQQTPAQVALAWSVQRGVAFLTTSATLSHIQENFAISTLPHRAMLEIKDDITTRVRF NSVVETGVPGFIPGKK